ONECUT2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ONECUT2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IP, chIP, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

15

Cat no : 21916-1-AP

Synonyms

OC2, OC 2, HNF-6-beta, HNF6B, HNF 6 beta

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Validation Data Gallery

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  WB analysis using 21916-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21916-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 21916-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 21916-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21916-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 21916-1-AP

  IP result of anti-ONECUT2 (IP:21916-1-AP, 4ug; Detection:21916-1-AP 1:300) with HEK-293 cells lysate 3200ug.

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  IHC staining of human stomach cancer using 21916-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21916-1-AP (ONECUT2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, mouse skin tissue, HEK-293 cells, HepG2 cells, Jurkat cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IP: See 1 publications below
• ChIP: See 3 publications below

Product Information

21916-1-AP targets ONECUT2 in WB, IHC, IP, chIP, ELISA, Cell treatment applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ONECUT2 fusion protein Ag16162
• Full Name: one cut homeobox 2
• Calculated Molecular Weight: 504 aa, 54 kDa
• Observed Molecular Weight: 54-60 kDa
• GenBank Accession Number: BC108925
• Gene Symbol: ONECUT2
• Gene ID (NCBI): 9480
• RRID: AB_2848180
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ONECUT2, also named as One cut domain family member 2 or Hepatocyte nuclear factor 6-beta, is a 504 amino acid protein, which contains one CUT DNA-binding domain and one homeobox DNA-binding domain. ONECUT2 belongs to the CUT homeobox family and activates the transcription of a number of liver genes such as HNF3B. ONECUT2 is required for liver differnetiation and metabolism. ONECUT2 and ONECUT3 play redundant roles with ONECUT1 in pancreas development.

Publications

Species	Application	Title
human	WB	Cancer Cell; Temporal evolution reveals bifurcated lineages in aggressive neuroendocrine small cell prostate cancer trans-differentiation; Authors - Chia-Chun Chen
human	WB	Nat Med; ONECUT2 is a targetable master regulator of lethal prostate cancer that suppresses the androgen axis.; Authors - Mirja Rotinen
human	IHC	Cancer Cell; Epigenomic State Transitions Characterize Tumor Progression in Mouse Lung Adenocarcinoma.; Authors - Lindsay M LaFave
human,mouse	WB	Nat Commun; Neuronal miR-9 promotes HSV-1 epigenetic silencing and latency by repressing Oct-1 and Onecut family genes; Authors - Yue Deng
human	WB	Sci Adv; Hypoxia-mediated stabilization of HIF1A in prostatic intraepithelial neoplasia promotes cell plasticity and malignant progression.; Authors - Mohamed A Abu El Maaty
human	WB	Proc Natl Acad Sci U S A; Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.; Authors - Lei Shi ; KD Validated