OPA3 Monoclonal antibody

OPA3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

5A3F9

Cat no : 68241-1-Ig

Synonyms

FLJ22187, FLJ25932, MGA3, OPA3, Optic atrophy 3 protein

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Validation Data Gallery

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  WB analysis using 68241-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68241-1-Ig (OPA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68241-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68241-1-Ig (OPA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, K-562 cells, A431 cells, HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68241-1-Ig targets OPA3 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: OPA3 fusion protein Ag8173
• Full Name: optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia)
• Calculated Molecular Weight: 179 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC005059
• Gene Symbol: OPA3
• Gene ID (NCBI): 80207
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The OPA3 cDNA encodes a deduced 179-amino acid protein. Northern blot analysis demonstrated a primary transcript of approximately 5.0 kb that was ubiquitously expressed, most prominently in skeletal muscle and kidney. Mutations in this gene have been shown to result in 3-methylglutaconic aciduria type III and autosomal dominant optic atrophy and cataract.