Oligophrenin 1 Polyclonal antibody

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• Oligophrenin 1 Polyclonal antibody (17445-1-AP)

Oligophrenin 1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 17445-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Synonyms

MRX60, oligophrenin 1, OPHN1, OPN1

• 

  WB analysis using 17445-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17445-1-AP (Oligophrenin 1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse skeletal muscle using 17445-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17445-1-AP (Oligophrenin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 17445-1-AP

  IP result of anti-Oligophrenin 1 (IP:17445-1-AP, 4ug; Detection:17445-1-AP 1:500) with mouse brain tissue lysate 5200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 17445-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17445-1-AP (Oligophrenin 1 antibody at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 17445-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17445-1-AP (Oligophrenin 1 antibody at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 17445-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17445-1-AP (Oligophrenin 1 antibody at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 17445-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17445-1-AP (Oligophrenin 1 antibody at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse skeletal muscle tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17445-1-AP targets Oligophrenin 1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11538

  Product name: Recombinant human OPHN1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-311 aa of BC059393

  Sequence: MGHPPLEFSDCYLDSPDFRERLKCYEQELERTNKFIKDVIKDGNALISAMRNYSSAVQKFSQTLQSFQFDFIGDTLTDDEINIAESFKEFAELLNEVENERMMMVHNASDLLIKPLENFRKEQIGFTKERKKKFEKDGERFYSLLDRHLHLSSKKKESQLQEADLQVDKERHNFFESSLDYVYQIQEVQESKKFNIVEPVLAFLHSLFISNSLTVELTQDFLPYKQQLQLSLQNTRNHFSSTREEMEELKKRMKEAPQTCKLPGQPTIEGYLYTQEKCVWGHRGIHWVSISQELLPLVGCEFWAPLLFIDP

• Full Name: oligophrenin 1
• Calculated Molecular Weight: 802 aa, 92 kDa
• Observed Molecular Weight: 91 kDa
• GenBank Accession Number: BC059393
• Gene Symbol: Oligophrenin 1
• Gene ID (NCBI): 4983
• RRID: AB_2158304
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60890
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The OPHN1 gene encodes a Rho-GTPase activating protein (RhoGAP), and mutations in OPHN1 are responsible for non-specific X-linked mental retardation (NSMR). OPHN1 is highly expressed in the brain and present both pre- and postsynaptically in neurons.

Publications

What published studies show

The Oligophrenin 1 Polyclonal antibody (Cat# 17445-1-AP) has 1 total citation. It was cited in Cell Reports in a study investigating error-prone protein synthesis and its role in recapitulating early symptoms of Alzheimer's disease in aging mice. The research field is neurodegeneration, specifically Alzheimer's disease. The antibody was used for Western blot in mouse samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Rep; Error-prone protein synthesis recapitulates early symptoms of Alzheimer disease in aging mice; Authors - Margarita Brilkova