OPLAH Polyclonal antibody

OPLAH Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21127-1-AP

Synonyms

5 Opase, 5 oxo L prolinase, 5 oxoprolinase, OPLA, OPLAH, Pyroglutamase

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Validation Data Gallery

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  WB analysis using 21127-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21127-1-AP (OPLAH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 21127-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21127-1-AP (OPLAH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse bladder using 21127-1-AP

  mouse bladder tissue were subjected to SDS PAGE followed by western blot with 21127-1-AP (OPLAH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21127-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21127-1-AP (OPLAH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse brain tissue, L02 cells, mouse bladder tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 21127-1-AP is OPLAH Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OPLAH fusion protein Ag15523
• Full Name: 5-oxoprolinase (ATP-hydrolysing)
• Calculated Molecular Weight: 1288 aa, 137 kDa
• Observed Molecular Weight: 135-140 kDa
• GenBank Accession Number: BC142672
• Gene Symbol: OPLAH
• Gene ID (NCBI): 26873
• RRID: AB_10733639
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	FASEB J; Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism.; Authors - Kenneth N Maclean