OPLAH Polyclonal antibody

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• OPLAH Polyclonal antibody (21127-1-AP)

OPLAH Polyclonal Antibody for WB, ELISA

Cat No. 21127-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

5 Opase, 5 oxo L prolinase, 5 oxoprolinase, OPLA, OPLAH, Pyroglutamase

• 

  WB analysis using 21127-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21127-1-AP (OPLAH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 21127-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21127-1-AP (OPLAH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of L02 using 21127-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21127-1-AP (OPLAH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse bladder using 21127-1-AP

  mouse bladder tissue were subjected to SDS PAGE followed by western blot with 21127-1-AP (OPLAH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse brain tissue, L02 cells, mouse bladder tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21127-1-AP targets OPLAH in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15523

  Product name: Recombinant human OPLAH protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 939-1288 aa of BC142672

  Sequence: ELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFGTSRQARGLPLEVSSEDHMDDGSPIRLRVQISLSQGSAVFDFSGTGPEVFGNLNAPRAVTLSALIYCLRCLVGRDIPLNQGCLAPVRVVIPRGSILDPSPEAAVVGGNVLTSQRVVDVILGAFGACAASQGCMNNVTLGNAHMGYYETVAGGAGAGPSWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRRGSGGRGRFRGGDGVTRELLFREEALLSVLTERRAFRPYGLHGGEPGARGLNLLIRKNGRTVNLGGKTSVTVYPGDVFCLHTPGGGGYGDPEDPAPPPGSPPQALAFPEHGSVYEYRRAQEAV

• Full Name: 5-oxoprolinase (ATP-hydrolysing)
• Calculated Molecular Weight: 1288 aa, 137 kDa
• Observed Molecular Weight: 135-140 kDa
• GenBank Accession Number: BC142672
• Gene Symbol: OPLAH
• Gene ID (NCBI): 26873
• RRID: AB_10733639
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14841
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The OPLAH Polyclonal Antibody (Cat# 21127-1-AP) has 2 total citations. One appears in FASEB Journal (2017), studying taurine treatment and hepatic γ-glutamyl cycle/methylglyoxal metabolism in a mouse model of classical homocystinuria, using WB in mouse samples. The other is in Clinical and Experimental Medicine (2026), investigating PYCR1's role in glutamine metabolism and lung adenocarcinoma progression via OPLAH regulation. The research fields span metabolic disorders and oncology.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	FASEB J; Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism.; Authors - Kenneth N Maclean
		Clin Exp Med; PYCR1 promotes glutamine metabolism and the progression of lung adenocarcinoma by regulating the expression of OPLAH.; Authors - Wei Chen