Validation Data Gallery
|Positive WB detected in||Jurkat cells, mouse ovary tissue, PC-3 cells, A375 cells, mouse spleen tissue|
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
13130-1-AP targets ORAI1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ORAI1 fusion protein Ag3758|
|Full Name||ORAI calcium release-activated calcium modulator 1|
|Calculated molecular weight||301 aa, 33 kDa|
|Observed molecular weight||45-50 kDa|
|GenBank accession number||BC015369|
|Gene ID (NCBI)||84876|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.
Direct control of store-operated calcium channels by ultrafast laser.
Orai1 (CRACM1) is the platelet SOC channel and essential for pathological thrombus formation.
The serum- and glucocorticoid-inducible kinase 1 (SGK1) influences platelet calcium signaling and function by regulation of Orai1 expression in megakaryocytes.
AMP-activated kinase is a regulator of fibroblast growth factor 23 production.
Adhesion to laminin-1 and collagen IV induces the formation of Ca2+ microdomains that sensitize mouse T cells for activation
ORAI1, STIM1/2, and RYR1 shape subsecond Ca2+ microdomains upon T cell activation.