|Positive WB detected in||Jurkat cells, mouse ovary tissue, PC-3 cells, A375 cells, mouse spleen tissue|
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
13130-1-AP targets ORAI1 in WB, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ORAI1 fusion protein Ag3758|
|Full Name||ORAI calcium release-activated calcium modulator 1|
|Calculated molecular weight||301 aa, 33 kDa|
|Observed molecular weight||45-50 kDa|
|GenBank accession number||BC015369|
|Gene ID (NCBI)||84876|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.
FUN14 domain-containing 1 promotes breast cancer proliferation and migration by activating calcium-NFATC1-BMI1 axis.
Biochem Biophys Res Commun
Upregulation of Orai1 and STIM1 expression as well as store-operated Ca2+ entry in ovary carcinoma cells by placental growth factor.
ORAI1, STIM1/2, and RYR1 shape subsecond Ca2+ microdomains upon T cell activation.
Store-operated Ca2+ entry in rhabdomyosarcoma cells
SOCE proteins, STIM1 and Orai1, are localized to the cleavage furrow during cytokinesis of the first and second cell division cycles in zebrafish embryos
The protective effects of curcumin in cerebral ischemia and reperfusion injury through PKC-θ signaling.