ORAI1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ORAI1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

43

Cat no : 13130-1-AP

Synonyms

CRACM1, ORAI1 L1, ORAI1 L2, Orail L2, ORAT1, Protein orai 1, TMEM142A, Transmembrane protein 142A

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Validation Data Gallery

• 

  WB analysis of Jurkat using 13130-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13130-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 13130-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 13130-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13130-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 13130-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 13130-1-AP

  Immunofluorescent analysis of HEK-293 cells using 13130-1-AP (ORAI1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse ovary tissue, PC-3 cells, A375 cells, mouse spleen tissue
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 35 publications below
• IF: See 8 publications below

Product Information

13130-1-AP targets ORAI1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ORAI1 fusion protein Ag3758
• Full Name: ORAI calcium release-activated calcium modulator 1
• Calculated Molecular Weight: 301 aa, 33 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC015369
• Gene Symbol: ORAI1
• Gene ID (NCBI): 84876
• RRID: AB_2157558
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.

Publications

Species	Application	Title
human	WB,IF	Cell Res; Direct control of store-operated calcium channels by ultrafast laser.; Authors - Pan Cheng ; KO Validated
mouse	WB	Blood; Orai1 (CRACM1) is the platelet SOC channel and essential for pathological thrombus formation.; Authors - Braun Attila A
mouse	WB	Blood; The serum- and glucocorticoid-inducible kinase 1 (SGK1) influences platelet calcium signaling and function by regulation of Orai1 expression in megakaryocytes.; Authors - Borst Oliver O
mouse	WB	Kidney Int; AMP-activated kinase is a regulator of fibroblast growth factor 23 production.; Authors - Philipp Glosse
mouse	IF	Sci Signal; Adhesion to laminin-1 and collagen IV induces the formation of Ca2+ microdomains that sensitize mouse T cells for activation; Authors - Mariella Weiß
mouse	IF	Sci Signal; ORAI1, STIM1/2, and RYR1 shape subsecond Ca2+ microdomains upon T cell activation.; Authors - Björn-Philipp Diercks

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