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ORAI1 Polyclonal antibody

ORAI1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 13130-1-AP

Synonyms

CRACM1, ORAI1 L1, ORAI1 L2, Orail L2, ORAT1, Protein orai 1, TMEM142A, Transmembrane protein 142A



Tested Applications

Positive WB detected inJurkat cells, mouse ovary tissue, PC-3 cells, A375 cells, mouse spleen tissue
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

13130-1-AP targets ORAI1 in WB, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ORAI1 fusion protein Ag3758
Full Name ORAI calcium release-activated calcium modulator 1
Calculated molecular weight 301 aa, 33 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC015369
Gene symbol ORAI1
Gene ID (NCBI) 84876
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.

Protocols

Product Specific Protocols
WB protocol for ORAI1 antibody 13130-1-APDownload protocol
IF protocol for ORAI1 antibody 13130-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EBioMedicine

FUN14 domain-containing 1 promotes breast cancer proliferation and migration by activating calcium-NFATC1-BMI1 axis.

Authors - Lianpin Wu
humanWB

Biochem Biophys Res Commun

Upregulation of Orai1 and STIM1 expression as well as store-operated Ca2+ entry in ovary carcinoma cells by placental growth factor.

Authors - Khalid N M Abdelazeem
mouseIF

Sci Signal

ORAI1, STIM1/2, and RYR1 shape subsecond Ca2+ microdomains upon T cell activation.

Authors - Björn-Philipp Diercks
humanWB

Store-operated Ca2+ entry in rhabdomyosarcoma cells

Authors -
zebrafishIF

SOCE proteins, STIM1 and Orai1, are localized to the cleavage furrow during cytokinesis of the first and second cell division cycles in zebrafish embryos

Authors -
ratWB

Cell Cycle

The protective effects of curcumin in cerebral ischemia and reperfusion injury through PKC-θ signaling.

Authors - Yun Mo