ORAI1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ORAI1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (2)

Applications

WB, IHC, IF

Conjugate

Unconjugated

Publications

5

Cat no : 28411-1-AP

Synonyms

CRACM1, FLJ14466, ORAI1, ORAI1 L1, ORAI1 L2, Orail L2, ORAT1, Protein orai 1, TMEM142A, Transmembrane protein 142A

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Validation Data Gallery

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  WB analysis using 28411-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 28411-1-AP (ORAI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 28411-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 28411-1-AP (ORAI1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 28411-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28411-1-AP (ORAI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 28411-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28411-1-AP (ORAI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MDA-MB-453s cells, MCF-7 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Product Information

28411-1-AP targets ORAI1 in WB, IHC, IF applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ORAI1 fusion protein Ag29234
• Full Name: ORAI calcium release-activated calcium modulator 1
• Calculated Molecular Weight: 301 aa, 33 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC015369
• Gene Symbol: ORAI1
• Gene ID (NCBI): 84876
• RRID: AB_2918160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. ORAI1 is a complex glycosylated protein, it exists in several molecular weight forms: a theoretical molecular weight of 32.7 kDa of the unmodified protein and two major glycosylated proteins of ~43 kDa and ~50 kDa (PMID: 22318387). Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.

Publications

Species	Application	Title
human	WB	Front Oncol; Calcium Channel Protein ORAI1 Mediates TGF-β Induced Epithelial-to-Mesenchymal Transition in Colorectal Cancer Cells.; Authors - Qingjie Kang ; KD Validated
human	WB	J Mol Med (Berl); Phosphate-induced ORAI1 expression and store-operated Ca2+ entry in aortic smooth muscle cells.; Authors - Ke Ma
mouse	WB,IF	Life Sci; The role of STIM1/ORAI1 channel in the analgesic effect of grain-sized moxibustion on inflammatory pain mice model.; Authors - Cheng-Shun Zhang
bovine	WB	J Dairy Sci; Role of ORAI calcium release-activated calcium modulator 1 (ORAI1) on neutrophil extracellular trap formation in dairy cows with subclinical hypocalcemia.; Authors - Bingbing Zhang ; KD Validated
bovine	IF	J Anim Sci; Intracellular Ca2+ signaling and ORAI calcium release-activated calcium modulator 1 (ORAI1) are associated with hepatic lipidosis in dairy cattle.; Authors - Ming Li