Tested Applications
| Positive WB detected in | HL-60 cells, 4T1 cells, A375 cells, RAW 264.7 cells, pig spleen tissue, rat spleen tissue, pig thymus tissue, Raji cells, Ramos cells, Daudi cells, Jurkat cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:30000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 23 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
Product Information
66223-1-Ig targets ORAI1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, monkey, bovine |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18050 Product name: Recombinant human ORAI1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-301 aa of BC015369 Sequence: MHPEPAPPPSRSSPELPPSGGSTTSGSRRSRRRSGDGEPPGAPPPPPSAVTYPDWIGQSYSEVMSLNEHSMQALSWRKLYLSRAKLKASSRTSALLSGFAMVAMVEVQLDADHDYPPGLLIAFSACTTVLVAVHLFALMISTCILPNIEAVSNVHNLNSVKESPHERMHRHIELAWAFSTVIGTLLFLAEVVLLCWVKFLPLKKQPGQPRPTSKPPASGAAANVSTSGITPGQAAAIASTTIMVPFGLIFIVFAVHFYRSLVSHKTDRQFQELNELAEFARLQDQLDHRGDHPLTPGSHYA Predict reactive species |
| Full Name | ORAI calcium release-activated calcium modulator 1 |
| Calculated Molecular Weight | 301 aa, 33 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC015369 |
| Gene Symbol | ORAI1 |
| Gene ID (NCBI) | 84876 |
| RRID | AB_2881614 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96D31 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ORAI1 antibody 66223-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats | ||
Acta Pharmacol Sin Endothelial cell Orai1 is essential for endothelium-dependent contraction of mouse carotid arteries in normotensive and hypertensive mice | ||
Oncogenesis Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth | ||
Int J Mol Sci Neuroprotective Effect of Antiapoptotic URG7 Protein on Human Neuroblastoma Cell Line SH-SY5Y | ||
Front Cell Dev Biol Transmembrane Protein Ttyh1 Maintains the Quiescence of Neural Stem Cells Through Ca2+/NFATc3 Signaling. | ||
J Innate Immun Mannan-Binding Lectin Reduces Epithelial-Mesenchymal Transition in Pulmonary Fibrosis via Inactivating the Store-Operated Calcium Entry Machinery. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Udesh (Verified Customer) (01-19-2023) | The Ab worked well for WB. 20ug protein loaded. Probed overnight at 4 deg.
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