Tested Applications
| Positive WB detected in | HL-60 cells, 4T1 cells, A375 cells, RAW 264.7 cells, pig spleen tissue, rat spleen tissue, pig thymus tissue, Raji cells, Ramos cells, Daudi cells, Jurkat cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:30000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66223-1-Ig targets ORAI1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, pig, monkey, chicken, bovine, sheep |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18050 Product name: Recombinant human ORAI1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-301 aa of BC015369 Sequence: MHPEPAPPPSRSSPELPPSGGSTTSGSRRSRRRSGDGEPPGAPPPPPSAVTYPDWIGQSYSEVMSLNEHSMQALSWRKLYLSRAKLKASSRTSALLSGFAMVAMVEVQLDADHDYPPGLLIAFSACTTVLVAVHLFALMISTCILPNIEAVSNVHNLNSVKESPHERMHRHIELAWAFSTVIGTLLFLAEVVLLCWVKFLPLKKQPGQPRPTSKPPASGAAANVSTSGITPGQAAAIASTTIMVPFGLIFIVFAVHFYRSLVSHKTDRQFQELNELAEFARLQDQLDHRGDHPLTPGSHYA Predict reactive species |
| Full Name | ORAI calcium release-activated calcium modulator 1 |
| Calculated Molecular Weight | 301 aa, 33 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC015369 |
| Gene Symbol | ORAI1 |
| Gene ID (NCBI) | 84876 |
| RRID | AB_2881614 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96D31 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ORAI1 antibody 66223-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ORAI1 antibody (Cat# 66223-1-Ig) has accumulated 36 citations across journals including Advanced Science, Cell Death & Disease, Acta Pharmacologica Sinica, Oncogenesis, FASEB Journal, FEBS Journal, PLoS Pathogens, and Journal of Dairy Science. Research fields span calcium signaling and store-operated calcium entry (SOCE), ER stress, cardiovascular disease, neurodegeneration, cancer, inflammation, fibrosis, pulmonary hypertension, diabetes, rheumatoid arthritis, viral infections, and animal/dairy science.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Activation of Lysosomal Retrograde Transport Triggers TPC1-IP3R1 Ca2+ Crosstalk at Lysosome-ER MCSs Leading to Lethal Depleting of ER Calcium | ||
Adv Sci (Weinh) Single-Cell Dissection of Therapy-Induced Remodeling Uncovers a Fibroblast-Driven Immunosuppressive Niche and Targetable Vulnerabilities in Lethal Prostate Cancer. | ||
Cell Death Dis Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats | ||
Acta Pharmacol Sin Endothelial cell Orai1 is essential for endothelium-dependent contraction of mouse carotid arteries in normotensive and hypertensive mice | ||
J Transl Med Targeting unfolded protein response reverts ER stress and ER Ca2+ homeostasis in cardiomyocytes expressing the pathogenic variant of Lamin A/C R321X |
Reviews
What customers say
Both reviews are 5-star ratings for Western Blot applications. One reviewer reported the antibody performed well using a 1:2000 dilution in 5% NFDM with 20 µg protein loaded and an overnight probe at 4 °C. The other reviewer simply noted fast delivery. Overall, customers express satisfaction with the product's performance and shipping speed.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Alexis (Verified Customer) (04-22-2026) | Livraison rapide
|
Udesh (Verified Customer) (01-19-2023) | The Ab worked well for WB. 20ug protein loaded. Probed overnight at 4 deg.
 |
