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Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells, mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27598-1-AP targets ORMDL3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26253 Product name: Recombinant human ORMDL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-100 aa of BC071833 Sequence: MNVGTAHSEVNPNTRVMNSRGIWLSYVLAIGLLHIVLLSIPFVSVPVVWTLTNLIHNMGMYIFLHTVKGTPFETPDQGKARLLTHWEQMDYGVQFTASRK Predict reactive species |
| Full Name | ORM1-like 3 (S. cerevisiae) |
| Calculated Molecular Weight | 153 aa, 17 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC071833 |
| Gene Symbol | ORMDL3 |
| Gene ID (NCBI) | 94103 |
| RRID | AB_3742242 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N138 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ORMDL3 (ORM1-like 3) is an endoplasmic-reticulum (ER)-resident transmembrane protein that negatively regulates serine palmitoyl-transferase (SPT), the first and rate-limiting enzyme of de-novo sphingolipid biosynthesis, thereby controlling cellular ceramide levels. (PMID: 31376405)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ORMDL3 antibody 27598-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
