ORP1L Polyclonal antibody

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  WB analysis using 25268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25268-1-AP (ORP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

ORP1L Polyclonal Antibody for WB, ELISA

Cat No. 25268-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ORP1, ORP 1, OSBPL1A, OSBPL1, OSBP8

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25268-1-AP targets ORP1L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: oxysterol binding protein-like 1A
• Calculated Molecular Weight: 108 kDa
• Observed Molecular Weight: 50 kDa, 105-110 kDa
• GenBank Accession Number: NM_018030
• Gene Symbol: OSBPL1A
• Gene ID (NCBI): 114876
• RRID: AB_3669468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BXW6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ORP1L (Oxysterol-binding protein-related protein 1L) is a lipid transfer protein that belongs to the family of OSBP-related proteins (ORPs). It is recruited to lysosomes via its ankyrin repeat domains that bind to Rab7(PMID: 34504082). ORP1L also possesses an FFAT motif, which allows it to interact with the ER resident VAP proteins to aid in establishing the ER-lysosomes contacts. ORP1L is expressed in various tissues, with particularly high expression in the brain, lungs, and macrophages. ORP1L plays a crucial role in cholesterol homeostasis. Disruption of ORP1L function can lead to a block in cholesterol transport to the ER, resulting in the accumulation of cholesterol-enriched LELs in the Golgi/perinuclear region. This can disrupt cellular cholesterol balance, potentially contributing to various metabolic diseases. This suggests that ORP1L is necessary for the normal functioning of cholesterol export from the endo-lysosomal system to the ER, and its dysfunction could be implicated in the progression of diseases related to cholesterol metabolism dysregulation(PMID: 28564600). The molecular weight of ORP1L is 50 kDa.