• Featured Product
  • KD/KO Validated

OSBP Polyclonal antibody

OSBP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11096-1-AP

Synonyms

OSBP, OSBP1, oxysterol binding protein, Oxysterol binding protein 1



Tested Applications

Positive WB detected inHEK-293 cells, rat kidney tissue, mouse kidney tissue, HeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11096-1-AP targets OSBP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, yeast
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OSBP fusion protein Ag1578
Full Name oxysterol binding protein
Calculated molecular weight 89 kDa
Observed molecular weight 89 kDa
GenBank accession numberBC011581
Gene symbol OSBP
Gene ID (NCBI) 5007
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for OSBP antibody 11096-1-APDownload protocol
IHC protocol for OSBP antibody 11096-1-APDownload protocol
IF protocol for OSBP antibody 11096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nature

A phosphoinositide signalling pathway mediates rapid lysosomal repair

Authors - Jay Xiaojun Tan
humanWB,IF

Gastroenterology

Oxysterol-Binding Protein Is a Phosphatidylinositol 4-Kinase Effector Required for HCV Replication Membrane Integrity and Cholesterol Trafficking.

Authors - Hongliang Wang
humanWB

Cell Host Microbe

The antiviral effector IFITM3 disrupts intracellular cholesterol homeostasis to block viral entry.

Authors - Samad Amini-Bavil-Olyaee
humanWB,IF

PLoS Pathog

Modulation of the Host Lipid Landscape to Promote RNA Virus Replication: The Picornavirus Encephalomyocarditis Virus Converges on the Pathway Used by Hepatitis C Virus.

Authors - Cristina M Dorobantu
  • KD Validated
yeastWB

Hum Mol Genet

Molecular partners of hNOT/ALG3, the human counterpart of the Drosophila NOT and yeast ALG3 gene, suggest its involvement in distinct cellular processes relevant to congenital disorders of glycosylation, cancer, neurodegeneration and a variety of further pathologies.

Authors - Benedikt Hacker
humanIF

Mol Biol Cell

Regulation of oxysterol-binding protein Golgi localization through protein kinase D-mediated phosphorylation.

Authors - Nhek Sokha S

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (09-12-2019)

This antibody could detect endogenous OSBP protein both in WB and IF.

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: MEF