OSBP Polyclonal antibody

• Featured Product
• KD/KO Validated

OSBP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

19

Reviews (1)

Cat no : 11096-1-AP

Synonyms

Oxysterol-binding protein 1, Oxysterol binding protein 1, oxysterol binding protein, OSBP1

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Validation Data Gallery

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  WB analysis using 11096-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11096-1-AP (OSBP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 11096-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 11096-1-AP (OSBP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11096-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11096-1-AP (OSBP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa cells using 11096-1-AP

  IP result of anti-OSBP (11096-1-AP for IP and Detection).

• 

  IHC staining of human ovary tumor using 11096-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11096-1-AP (OSBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 11096-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11096-1-AP (OSBP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, rat kidney tissue, mouse kidney tissue, HeLa cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 16 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

11096-1-AP targets OSBP in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rabbit, yeast
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OSBP fusion protein Ag1578
• Full Name: oxysterol binding protein
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 89 kDa
• GenBank Accession Number: BC011581
• Gene Symbol: OSBP
• Gene ID (NCBI): 5007
• RRID: AB_2158220
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
human	WB,IF	Gastroenterology; Oxysterol-Binding Protein Is a Phosphatidylinositol 4-Kinase Effector Required for HCV Replication Membrane Integrity and Cholesterol Trafficking.; Authors - Hongliang Wang
human	WB	Cell Host Microbe; The antiviral effector IFITM3 disrupts intracellular cholesterol homeostasis to block viral entry.; Authors - Samad Amini-Bavil-Olyaee
human	WB,IF	PLoS Pathog; Modulation of the Host Lipid Landscape to Promote RNA Virus Replication: The Picornavirus Encephalomyocarditis Virus Converges on the Pathway Used by Hepatitis C Virus.; Authors - Cristina M Dorobantu ; KD Validated
		Int J Mol Sci; A Small Molecule, 4-Phenylbutyric Acid, Suppresses HCV Replication via Epigenetically Induced Hepatic Hepcidin.; Authors - Kiyoon Kim
yeast	WB	Hum Mol Genet; Molecular partners of hNOT/ALG3, the human counterpart of the Drosophila NOT and yeast ALG3 gene, suggest its involvement in distinct cellular processes relevant to congenital disorders of glycosylation, cancer, neurodegeneration and a variety of further pathologies.; Authors - Benedikt Hacker

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (09-12-2019)

This antibody could detect endogenous OSBP protein both in WB and IF.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: MEF