OSBPL10 Polyclonal antibody

OSBPL10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15491-1-AP

Synonyms

OSBP-related protein 10, OSBP9, OSBP related protein 10, ORP-10, ORP10

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Validation Data Gallery

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  WB analysis of HepG2 using 15491-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15491-1-AP (OSBPL10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa cells using 15491-1-AP

  IP result of anti-OSBPL10 (15491-1-AP for IP and Detection).

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  IHC staining of human colon cancer using 15491-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15491-1-AP (OSBPL10 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 15491-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15491-1-AP (OSBPL10 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 15491-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using OSBPL10 antibody (15491-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

15491-1-AP targets OSBPL10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OSBPL10 fusion protein Ag7861
• Full Name: oxysterol binding protein-like 10
• Calculated Molecular Weight: 84 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC003168
• Gene Symbol: OSBPL10
• Gene ID (NCBI): 114884
• RRID: AB_2158356
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OSBPL10, also known as ORP10, is a member of the oxysterol-binding protein family. OSBPL10 localizes on microtubules and acts as a regulator of cellular lipid biosyntheses and apolipoprotein B-100. Genetic variation in OSBPL10 is associated with dyslipidmias and peripheral artery disease.

Publications

Species	Application	Title
human	WB	Biochim Biophys Acta; ORP10, a cholesterol binding protein associated with microtubules, regulates apolipoprotein B-100 secretion.; Authors - Nissil? Eija E
human	IF,WB	Nat Commun; Regulation of cellular cholesterol distribution via non-vesicular lipid transport at ER-Golgi contact sites; Authors - Tomoki Naito