OSBPL9 Polyclonal antibody

OSBPL9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11879-1-AP

Synonyms

ORP 9, ORP9, OSBP related protein 9, OSBP4, OSBPL9

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Validation Data Gallery

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  WB analysis of human brain using 11879-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11879-1-AP (OSBPL9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11879-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11879-1-AP (OSBPL9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11879-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11879-1-AP (OSBPL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11879-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11879-1-AP (OSBPL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 11879-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11879-1-AP (OSBPL9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 11879-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11879-1-AP (OSBPL9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 11879-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11879-1-AP (OSBPL9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 11879-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11879-1-AP (OSBPL9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 11879-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11879-1-AP (OSBPL9 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, human testis tissue, mouse lung tissue
• Positive IHC detected in: human testis tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

11879-1-AP targets OSBPL9 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OSBPL9 fusion protein Ag2476
• Full Name: oxysterol binding protein-like 9
• Calculated Molecular Weight: 736 aa, 83 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC025978
• Gene Symbol: OSBPL9
• Gene ID (NCBI): 114883
• RRID: AB_10638005
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ORP9 is expressed as a full-length (ORP9L) or truncated version missing the PH domain (ORP9S).

Publications

Species	Application	Title
human	WB	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
human	WB	Cell Mol Life Sci; ORP9 and ORP10 form a heterocomplex to transfer phosphatidylinositol 4-phosphate at ER-TGN contact sites; Authors - Ruyue He
human	WB	J Cell Biol; Phosphatidylserine regulates plasma membrane repair through tetraspanin-enriched macrodomains; Authors - Yang E Li ; KO Validated
human		Elife; The ORP9-ORP11 dimer promotes sphingomyelin synthesis; Authors - Birol Cabukusta