Tested Applications
| Positive WB detected in | A549 cells, mouse liver tissue, mouse ovary tissue, rat ovary tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15248-1-AP targets OSGIN1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4295 Product name: Recombinant human OSGIN1 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 130-477 aa of BC032476 Sequence: AWHSIEGSMVILSQGQWMGLPDLEVKDWMQKKRRGLRNSRATAGDIAHYYRDYVVKKGLGHNFVSGAVVTAVEWGTPDPSSCGAQDSSPLFQVSGFLTRNQAQQPFSLWARNVVLATGTFDSPARLGIPGEALPFIHHELSALEAATRVGAVTPASDPVLIIGAGLSAADAVLYARHYNIPVIHAFRRAVDDPGLVFNQLPKMLYPEYHKVHQMMREQSILSPSPYEGYRSLPRHQLLCFKEDCQAVFQDLEGVEKVFGVSLVLVLIGSHPDLSFLPGAGADFAVDPDQPLSAKRNPIDVDPFTYQSTRQEGLYAMGPLAGDNFVRFVQGGALAVASSLLRKETRKPP Predict reactive species |
| Full Name | oxidative stress induced growth inhibitor 1 |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC032476 |
| Gene Symbol | OSGIN1 |
| Gene ID (NCBI) | 29948 |
| RRID | AB_2156401 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UJX0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
OSGIN1, also named as BDGI and OKL38, belongs to the OKL38 family. OSGIN1 is a gene linked to oxidative stress and cell death in other tissues, was markedly upregulated in smokers compared to nonsmokers (PMID: 28548877). OSGIN1 has 3 isoforms with the molecular mass of 38, 52 and 61 kDa (PMID: 28181536).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OSGIN1 antibody 15248-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The OSGIN1 Polyclonal antibody (Cat# 15248-1-AP) has 8 citations published in journals including Journal of Hazardous Materials, International Journal of Nanomedicine, Free Radical Biology and Medicine, Cell & Molecular Life Sciences, International Journal of Molecular Sciences, Journal of Pharmacology and Experimental Therapeutics, and Toxicology Letters. Research fields span oxidative stress, ferroptosis, tumor progression in NSCLC, apoptosis in neural stem cells, melanoma, COPD-related fibrosis, autophagy, and photo-sonodynamic anti-tumor therapy.
| Species | Application | Title |
|---|---|---|
J Hazard Mater METTL3 regulates PM2.5-induced cell injury by targeting OSGIN1 in human airway epithelial cells. | ||
Int J Nanomedicine In-Situ Grown Nanocrystal TiO2 on 2D Ti3C2 Nanosheets with Anti-Tumor Activity from Photo-Sonodynamic Treatment and Immunology | ||
Free Radic Biol Med Radiotherapy disrupts ferroptosis tolerance by reducing DNMT1 levels and uncouples STING silencing in LKB1-deficient lung tumors. | ||
Cell Mol Life Sci OSGIN1 is a novel TUBB3 regulator that promotes tumor progression and gefitinib resistance in non-small cell lung cancer | ||
Int J Mol Sci Methylmercury Induces Apoptosis in Mouse C17.2 Neural Stem Cells through the Induction of OSGIN1 Expression by NRF2 | ||
J Pharmacol Exp Ther Mechanistic Analysis of an Extracellular Signal-Regulated Kinase 2-Interacting Compound that Inhibits Mutant BRAF-Expressing Melanoma Cells by Inducing Oxidative Stress. |
