OSTF1 Polyclonal antibody

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  WB analysis of C2C12 using 10671-1-AP

  C2C12 cells were subjected to SDS PAGE followed by western blot with 10671-1-AP (OSTF1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis using 10671-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10671-1-AP (OSTF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10671-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10671-1-AP (OSTF1 antibody) at dilution of 1:100 (under 25x lens).

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  IHC staining of human lung using 10671-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 10671-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 10671-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10671-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 10671-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 10671-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 10671-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10671-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 10671-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using OSTF1 antibody (10671-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

OSTF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10671-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

OSF, Osteoclast-stimulating factor 1, SH3P2

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Tested Applications

• Positive WB detected in: HeLa cells, C2C12 cells, HepG2 cells, U-937 cells
• Positive IHC detected in: human breast cancer tissue, human kidney tissue, human lung tissue, human placenta tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

10671-1-AP targets OSTF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1072

  Product name: Recombinant human OSTF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-217 aa of BC007459

  Sequence: MSKPPPKPVKPGEGGQVKVFRALYTFEPRTPDELYFEEGDIIYITDMSDTNWWKGTSKGRTGLIPSNYVAEQAESIDNPLHEAAKRGNLSWLRECLDNRVGVNGLDKAGSTALYWACHGGHKDIVEMLFTQPNIELNQQNKLGDTALHAAAWKGYADIVQLFLAKGARTDLRNIEKKLAFDMATNAACASLLKKKQGTDAVRTLSNAEDYLDDEDSD

• Full Name: osteoclast stimulating factor 1
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC007459
• Gene Symbol: OSTF1
• Gene ID (NCBI): 26578
• RRID: AB_2156717
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92882
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OSTF1 (Osteoclast-stimulating factor-1) is an intracellular protein produced by osteoclasts that indirectly induces osteoclast formation and bone resorption. It induces bone resorption, acting probably through a signaling cascade which results in the secretion of factor(s) enhancing osteoclast formation and activity

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat,human	WB,IHC	Heliyon; OSTF1 knockdown mitigates IL-1β-induced chondrocyte injury via inhibiting the NF-κB signaling pathway; Authors - Bin Hu