Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12066-1-AP targets OTUB2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2703 Product name: Recombinant human OTUB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-73 aa of BC009615 Sequence: MSETSFNLISEKCDILSILRDHPENRIYRRKIEELSKRFTAIRKTKGDGNCFYRALGYSYLESLLGKSREIFK Predict reactive species |
| Full Name | OTU domain, ubiquitin aldehyde binding 2 |
| Calculated Molecular Weight | 234 aa, 27 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC009615 |
| Gene Symbol | OTUB2 |
| Gene ID (NCBI) | 78990 |
| RRID | AB_3085388 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96DC9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
OTUB2, also known as C14orf137, belongs to the OTU family. OTUB2 is a functional cysteine protease with deubiquitinase activity. OTUB2 mediates the deubiquitination of substrate proteins, and the targets of substrate proteins can be diversified. Therefore, OTUB2 has a wide range of biological roles, including protecting against virus-induced activation of interferon regulatory factor 3 (IRF3) and NF-κB, promoting beta cell survival in human pancreatic islets, and supporting the DNA repair pathway (PMID: 30662561, 35309903). OTUB2 is widely expressed, with high expression levels in the brain. OTUB2 has 2 isoforms with molecular weights of 9 kDa and 27 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for OTUB2 antibody 12066-1-AP | Download protocol |
| WB protocol for OTUB2 antibody 12066-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-OTUB2 antibody (Cat# 12066-1-AP) has 6 citations published in journals including Nature Cell Biology, Acta Pharmaceutica Sinica B, Cell Death & Disease, Virus Sinica, Human Cell, and Aging. Research fields span Hippo signaling and mechanotransduction, chemoresistance in triple-negative breast cancer, autophagy-ferroptosis evasion in colorectal cancer, SARS-CoV-2 replication, pathological cardiac hypertrophy, and deubiquitinase-related signatures predicting breast cancer prognosis and immunotherapy response. Applications include WB and IF in human, mouse, and rat samples.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Microtubule architecture connects AMOT stability to YAP/TAZ mechanotransduction and Hippo signalling | ||
Acta Pharm Sin B USP51/GRP78/ABCB1 axis confers chemoresistance through decreasing doxorubicin accumulation in triple-negative breast cancer cells | ||
Cell Death Dis OTUB2-mediated deubiquitination upregulates U2AF2 to promote colorectal cancer evasion of autophagy-ferroptosis.
| ||
Hum Cell OTUB2 aggravates pathological cardiac hypertrophy through Rac1 activation.
| ||
Aging (Albany NY) The deubiquitinating enzymes-related signature predicts the prognosis and immunotherapy response in breast cancer
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