Tested Applications
| Positive WB detected in | HeLa cells, HCT 116 cells, HT-29 cells, HepG2 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29622-1-AP targets OTUD3 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30425 Product name: Recombinant human OTUD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 192-398 aa of NM_015207 Sequence: NSEAPAHLQTDFQMLHQDESNKREKIKTKGMDSEDDLRDEVEDAVQKVCNATGCSDFNLIVQNLEAENYNIESAIIAVLRMNQGKRNNAEENLEPSGRVLKQCGPLWEEGGSGARIFGNQGLNEGRTENNKAQASPSEENKANKNQLAKVTNKQRREQQWMEKKKRQEERHRHKALESRGSHRDNNRSEAEANTQVTLVKTFAALNI Predict reactive species |
| Full Name | OTU domain containing 3 |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | NM_015207 |
| Gene Symbol | OTUD3 |
| Gene ID (NCBI) | 23252 |
| RRID | AB_2918332 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5T2D3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
OTUD3 is a kind of deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. It's an important regulator of energy metabolism. Glucose and fatty acids trigger its nuclear translocation by CBP-dependent acetylation. In the nucleus, deubiquitinates and stabilizes the nuclear receptor PPARD regulating the expression of various genes involved in glucose and lipid metabolism and oxidative phosphorylation. The calculated MW of OTUD3 is 45 kDa, 29622-1-AP can detect the band around 43 kDa. (PubMed:23827681, 35675826).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OTUD3 antibody 29622-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The OTUD3 Polyclonal antibody (Cat# 29622-1-AP) has 5 total citations published in Nature Communications, Science China Life Sciences, Clinical and Translational Medicine, European Journal of Pharmacology, and Journal of Biological Chemistry. The research fields span ubiquitin code and deubiquitination mechanisms, protein modification in aflatoxin cytotoxicity, neuroprotection against ferroptosis in cerebral ischemia-reperfusion, ER-phagy in lung cancer therapy, and cGAS signaling regulation by E3 ligase-mediated degradation.
| Species | Application | Title |
|---|---|---|
Sci China Life Sci CRISPR screening identifies protein methylation and ubiquitination modifications that modulate aflatoxin B1 cytotoxicity | ||
Clin Transl Med The deubiquitinase OTUD3 plays a neuroprotective role by reducing ferroptosis induced by cerebral ischaemia reperfusion via stabilizing PLK1 via deubiquitination | ||
Eur J Pharmacol OTUD3-mediated deubiquitination licenses TEX264 to orchestrate ER-phagy for KDM5B degradation in teniposide lung cancer therapy | ||
J Biol Chem The E3 ligase β-TRCP1 earmarks OTUD3 for destruction to fine-tune cGAS activation. |
