OTUD4 Polyclonal antibody

• Featured Product
• KD/KO Validated

OTUD4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 25070-1-AP

Synonyms

DUBA6, HIN1, HIV 1 induced protein HIN 1, HSHIN1, KIAA1046, OTU domain containing 4, OTUD4

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Validation Data Gallery

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  WB analysis using 25070-1-AP

  Various lysate were subjected to SDS PAGE followed by western blot with 25070-1-AP (OTUD4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 25070-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25070-1-AP (OTUD4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 25070-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25070-1-AP (OTUD4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Daudi cells, Raji cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Product Information

25070-1-AP targets OTUD4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OTUD4 fusion protein Ag18401
• Full Name: OTU domain containing 4
• Calculated Molecular Weight: 1113 aa, 124 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC118653
• Gene Symbol: OTUD4
• Gene ID (NCBI): 54726
• RRID: AB_2879882
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OTUD4, a member of the OTU family, encodes for a protein of 495 amino acids with an OTU domain and functions via their enzymatic deubiquitinating activities. OTUD4 nominally encodes a K48-specific deubiquitinase previously shown to counteract proteasomal degradation of DNA repair proteins. OTUD4 has been identified to be down-regulated in multiple human tumor types, and lower OTUD4 expression indicates a poor prognosis.(PMID: 36411454,PMID: 32184655,PMID: 29395066)

Publications

Species	Application	Title
human	WB	Cell Death Differ; N6-methyladenosine-modified USP13 induces pro-survival autophagy and imatinib resistance via regulating the stabilization of autophagy-related protein 5 in gastrointestinal stromal tumors; Authors - Zhishuang Gao
human	WB	Front Microbiol; MiR-103a-3p Promotes Zika Virus Replication by Targeting OTU Deubiquitinase 4 to Activate p38 Mitogen-Activated Protein Kinase Signaling Pathway.; Authors - Haiyan Ye ; KD Validated
human	WB,IHC,IF,IP	Exp Cell Res; Deubiquitinating enzyme OTUD4 regulates metastasis in triple-negative breast cancer by stabilizing Snail1; Authors - Xiuqing Ma ; KD Validated
human	WB	PLoS Pathog; Non-canonical regulation of the reactivation of an oncogenic herpesvirus by the OTUD4-USP7 deubiquitinases; Authors - Shaowei Wang
human	WB,IHC,CoIP	Cell Death Dis; OTUD4 promotes the progression of glioblastoma by deubiquitinating CDK1 and activating MAPK signaling pathway; Authors - Mingxin Ci ; KD Validated
human	WB,IHC	Biochem Biophys Res Commun; NRF2 regulates EGF stability through OTUD4 in lung adenocarcinoma; Authors - Shuming Hu