Tested Applications
| Positive WB detected in | HEK-293T cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells, C6 cells |
| Positive IHC detected in | rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21002-1-AP targets OTUD5 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15190 Product name: Recombinant human OTUD5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 202-561 aa of BC028225 Sequence: WFEKALRDKKGFIIKQMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRPVEVYQYSTEPINTFHGIHQNEDEPIRVSYHRNIHYNSVVNPNKATIGVGLGLPSFKPGFAEQSLMKNAIKTSEESWIEQQMLEDKKRATDWEATNEAIEEQVARESYLQWLRDQEKQARQVRGPSQPRKASATCSSATAAASSGLEEWTSRSPRQRSSASSPEHPELHAELGMKPPSPGTVLALAKPPSPCAPGTSSQFSAGADRATSPLVSLYPALECRALIQQMSPSAFGLNDWDDDEILASVLAVSQQEYLDSMKKNKVPCRC Predict reactive species |
| Full Name | OTU domain containing 5 |
| Calculated Molecular Weight | 571 aa, 61 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC028225 |
| Gene Symbol | OTUD5 |
| Gene ID (NCBI) | 55593 |
| RRID | AB_2878788 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96G74 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The deubiquitinating enzyme OTUD5, also known as deubiquitinating enzyme A (DUBA), is a member of the deubiquitinating enzyme OTU family. By deubiquitinating other proteins, OTUD5 is an important regulator of several physiological processes, such as immune signaling and DNA damage responses. The dysregulation of these processes can increase the risk of associated diseases, including inflammation, cancer and genetic disorders.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for OTUD5 antibody 21002-1-AP | Download protocol |
| IHC protocol for OTUD5 antibody 21002-1-AP | Download protocol |
| WB protocol for OTUD5 antibody 21002-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The OTUD5 Polyclonal antibody (Cat# 21002-1-AP) has 13 citations. These appear in journals including Nature Communications, Advanced Science, Free Radical Biology and Medicine, Clinical and Translational Medicine, Cell Reports, International Journal of Radiation Oncology, European Journal of Pharmacology, Carcinogenesis, Tissue Cell, eLife, and Experimental Molecular Medicine. Research fields span diabetic kidney disease, telomere repair, Parkinson's disease, hepatocellular and gastric cancers, septic cardiomyopathy, ferroptosis, radiation pneumonitis, lung adenocarcinoma, lung injury, breast cancer, and myocardial hypertrophy.
| Species | Application | Title |
|---|---|---|
Nat Commun Podocyte OTUD5 alleviates diabetic kidney disease through deubiquitinating TAK1 and reducing podocyte inflammation and injury | ||
Nat Commun OTUD5 promotes end-joining of deprotected telomeres by promoting ATM-dependent phosphorylation of KAP1S824 | ||
Adv Sci (Weinh) OTUD5 Protects Dopaminergic Neurons by Promoting the Degradation of α-Synuclein in Parkinson's Disease Model | ||
Free Radic Biol Med Saquinavir induces pyroptosis through the OTUD5-JAK1-GSDME axis in hepatocellular carcinoma.
| ||
Clin Transl Med Cardiomyocyte-enriched OTUD5 alleviates septic cardiomyopathy by promoting NLRP3 deubiquitination and inhibiting NLRP3 inflammasome activation. | ||
Clin Transl Med p53 inhibits OTUD5 transcription to promote GPX4 degradation and induce ferroptosis in gastric cancer
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