OTUD5 Polyclonal antibody

OTUD5 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 21002-1-AP

Synonyms

OTUD 5, OTU domain-containing protein 5, EC:3.4.19.12, DUBA

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Validation Data Gallery

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  WB analysis using 21002-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21002-1-AP (OTUD5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21002-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21002-1-AP (OTUD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 21002-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using OTUD5 antibody (21002-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells, C6 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

21002-1-AP targets OTUD5 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OTUD5 fusion protein Ag15190
• Full Name: OTU domain containing 5
• Calculated Molecular Weight: 571 aa, 61 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC028225
• Gene Symbol: OTUD5
• Gene ID (NCBI): 55593
• RRID: AB_2878788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	Int J Radiat Oncol Biol Phys; USP11 exacerbates radiation induced pneumonitis by activating endothelial cell inflammatory response via OTUD5-STING signaling; Authors - Yiting Tang
human	WB	Carcinogenesis; CacyBP promotes the development of lung adenocarcinoma by regulating OTUD5; Authors - Mixue Bai
mouse	WB,IF,CoIP	Nat Commun; Podocyte OTUD5 alleviates diabetic kidney disease through deubiquitinating TAK1 and reducing podocyte inflammation and injury; Authors - Ying Zhao
human	WB,IP	Eur J Pharmacol; Dapagliflozin suppressed gastric cancer growth via regulating OTUD5 mediated YAP1 deubiquitination; Authors - Kaijie Ren
human	WB	Elife; Hypoxia-induced proteasomal degradation of DBC1 by SIAH2 in breast cancer progression; Authors - Qiangqiang Liu