Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, PC-3 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25430-1-AP targets OTUD6B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22044 Product name: Recombinant human OTUD6B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-157 aa of BC029760 Sequence: MEAVLTEELDEEEQLLRRHRKEKKELQAKIQGMKNAVPKNDKKRRKQLTEDVAKLEKEMEQKHREELEQLKLTTKENKIDSVAVNISNLVLENQPPRISKAQKRREKKAALEKEREERIAEAEIENLTGARHMESEKLAQILAARQLEIKQIPSDGH Predict reactive species |
| Full Name | OTU domain containing 6B |
| Calculated Molecular Weight | 293 aa, 34 kDa |
| Observed Molecular Weight | 34-40 kDa |
| GenBank Accession Number | BC029760 |
| Gene Symbol | OTUD6B |
| Gene ID (NCBI) | 51633 |
| RRID | AB_2880075 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N6M0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for OTUD6B antibody 25430-1-AP | Download protocol |
| IHC protocol for OTUD6B antibody 25430-1-AP | Download protocol |
| WB protocol for OTUD6B antibody 25430-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The OTUD6B antibody (Cat# 25430-1-AP) has 7 citations published in journals including Cell Death & Disease, Cell Mol Life Sci, EMBO Reports, Cell Biol Int, Biochem Biophys Res Commun, and Cell Signal. Research fields span stress granule dynamics, colorectal cancer liver metastasis, pulmonary arterial hypertension, breast cancer survival, cholangiocarcinoma, ferroptosis in triple-negative breast cancer, and multiple myeloma. Applications include WB, IHC, IF, and IP.
| Species | Application | Title |
|---|---|---|
Cell Death Dis Proximity proteomics reveals OTUD6B regulation of stress granule dynamics through coalescence with VCP/p97.
| ||
Cell Death Dis OTUD6B-mediated K48 deubiquitination of FXR1 forms a positive feedback loop activating MEK2/ERK signaling in colorectal cancer liver metastasis.
| ||
Cell Mol Life Sci Otud6b induces pulmonary arterial hypertension by mediating the Calpain-1/HIF-1α signaling pathway
| ||
EMBO Rep OTUD6B regulates KIFC1-dependent centrosome clustering and breast cancer cell survival | ||
Cell Biol Int OTUD6B promotes cholangiocarcinoma growth by regulating STAT3 phosphorylation through deubiquitination of PTK2
| ||
Biochem Biophys Res Commun Calycosin triggers ferroptosis through modulation of the OTUD6B/GPX4 axis to suppress triple-negative breast cancer. |
Reviews
What customers say
The single review rates the antibody 3 out of 5 stars. The user found it works perfectly for western blotting but was unable to optimize it for immunofluorescence. Despite testing a range of dilutions (1:500–1:5000) and various blocking and fixing methods on BT549 cells, no difference was observed between knockdown and control conditions. Overall, the antibody performs well for WB but may require further optimization for IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Dorota (Verified Customer) (04-11-2019) | Antibody works perfectly for western blotting, however I could not optimise it for immunofluorescence. I tried series of dilutions and couple of different blocking/fixing methods, but there was no difference between knockdown and control conditions in any of them.
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