Filter: AllWBIF/ICCAffinity

WB analysis using 87372-1-RR (same clone as 87372-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 87372-1-RR (OTX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87372-1-PBS in a different storage buffer formulation.
IF Staining of Y79 using 87372-1-RR (same clone as 87372-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed Y79 cells using OTX2 antibody (87372-1-RR, Clone: 252762H3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87372-1-PBS in a different storage buffer formulation.
Affinity of 87372-1-RR
Biolayer interferometry (BLl) kinetic assays of 87372-1-RR against Human OTX2 were performed. The affinity constant is 0.132 nM.

OTX2 Recombinant monoclonal antibody, PBS Only

Name: OTX2 Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 87372-1-PBS-100UG

OTX2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 87372-1-PBS

Clone No.252762H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Homeobox protein OTX2, MCOPS5

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

87372-1-PBS targets OTX2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag4323 Product name: Recombinant human OTX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-297 aa of BC032579 Sequence: MMSYLKQPPYAVNGLSLTTSGMDLLHPSVGYPGPWASCPAATPRKQRRERTTFTRAQLDVLEALFAKTRYPDIFMREEVALKINLPESRVQVWFKNRRAKCRQQQQQQQNGGQNKVRPAKKKTSPAREVSSESGTSGQFTPPSSTSVPTIASSSAPVSIWSPASISPLSDPLSTSSSCMQRSYPMTYTQASGYSQGYAGSTSYFGGMDCGSYLTPMHHQLPGPGATLSPMGTNAVTSHLNQSPASLSTQGYGASSLGFNSTTDCLDYKDQTASWKLNFNADCLDYKDQTSSWKFQVL Predict reactive species
Full Name	orthodenticle homeobox 2
Calculated Molecular Weight	297 aa, 32 kDa
Observed Molecular Weight	31-35 kDa
GenBank Accession Number	BC032579
Gene Symbol	OTX2
Gene ID (NCBI)	5015
RRID	AB_3745491
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P32243
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.