OVOL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

OVOL1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 14082-1-AP

Synonyms

HOVO1, ovo like 1(Drosophila), OVOL1

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Validation Data Gallery

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  WB analysis using 14082-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14082-1-AP (OVOL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14082-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14082-1-AP (OVOL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14082-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14082-1-AP (OVOL1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below

Product Information

14082-1-AP targets OVOL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OVOL1 fusion protein Ag5210
• Full Name: ovo-like 1(Drosophila)
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC059408
• Gene Symbol: OVOL1
• Gene ID (NCBI): 5017
• RRID: AB_2157296
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Death Dis; HCC-derived exosomes elicit HCC progression and recurrence by epithelial-mesenchymal transition through MAPK/ERK signalling pathway.; Authors - Lu Chen
human	WB	Cell Death Dis; cPLA2α mediates TGF-β-induced epithelial-mesenchymal transition in breast cancer through PI3k/Akt signaling.; Authors - Lu Chen
human	WB	Commun Biol; αvβ3 Integrin induces partial EMT independent of TGF-β signaling.; Authors - Yoshinobu Kariya
mouse	WB	Mol Carcinog; Twist1 is required for the development of UVB-induced squamous cell carcinoma.; Authors - Fernando Eguiarte-Solomon
human	WB	PLoS One; Transcription factors OVOL1 and OVOL2 induce the mesenchymal to epithelial transition in human cancer.; Authors - Hernan Roca ; KD Validated
human	WB	J Dermatol; Skin colonization by Staphylococcus aureus in hemodialysis patients with pruritus and the effect of Staphylococcus aureus-secreted α-toxin on filaggrin expression; Authors - Yen-Yu Tsai