Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, NIH/3T3 cells, L02 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21055-1-AP targets OXA1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13819 Product name: Recombinant human OXA1L protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 319-438 aa of BC001669 Sequence: MYWLSSNLFSLVQVSCLRIPAVRTVLKIPQRVVHDLDKLPPREGFLESFKKGWKNAEMTRQLREREQRMRNQLELAARGPLRQTFTHNPLLQPGKDNPPNIPSSSSKPKSKYPWHDTLG Predict reactive species |
| Full Name | oxidase (cytochrome c) assembly 1-like |
| Calculated Molecular Weight | 437 aa, 49 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC001669 |
| Gene Symbol | OXA1L |
| Gene ID (NCBI) | 5018 |
| RRID | AB_10695769 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15070 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
OXA1L/Oxa1 is a member of the conserved Oxa1/YidC/Alb3 protein family and is required for cytochrome c oxidase assembly. The OXA1L protein was initially identified in yeast proteins involved in the biogenesis of the mitochondrial respiratory chain complexes. It locates in the inner membrane of mitochondria and may be involved in mediating the insertion of proteins from the matrix into the inner membrane.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for OXA1L antibody 21055-1-AP | Download protocol |
| IHC protocol for OXA1L antibody 21055-1-AP | Download protocol |
| WB protocol for OXA1L antibody 21055-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The OXA1L Polyclonal antibody (Cat# 21055-1-AP) has 18 citations spanning journals including Nature Communications, Cell Metabolism, EMBO Journal, EMBO Molecular Medicine, American Journal of Human Genetics, Cell Reports, Journal of Cell Science, Journal of Biological Chemistry, Human Molecular Genetics, Frontiers in Genetics, Aging, Methods in Molecular Biology, and bioRxiv. Research fields cover mitochondrial protein import, oxidative phosphorylation, mitophagy, mitochondrial disease, metabolic dysfunction, and cancer biology.
| Species | Application | Title |
|---|---|---|
Cell Metab Proteolytic cleavage of opa1 stimulates mitochondrial inner membrane fusion and couples fusion to oxidative phosphorylation. | ||
EMBO J A unified mechanism for mitochondrial damage sensing in PINK1-Parkin-mediated mitophagy. | ||
EMBO Mol Med OXA1L mutations cause mitochondrial encephalopathy and a combined oxidative phosphorylation defect.
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Reviews
What customers say
The single review for this antibody rates it 5 stars. The customer used it for Western Blot and reported a clean band at the expected size, indicating reliable specificity and performance. Overall, the feedback is positive, with the reviewer highlighting clear, specific results in their WB application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (04-28-2023) | clean band around the expected size
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