OXA1L Monoclonal antibody

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• KD/KO Validated

OXA1L Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

4A2D9

Cat no : 66128-1-Ig

Synonyms

Hsa, OXA1 like protein, OXA1Hs, OXA1L

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Validation Data Gallery

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  WB analysis using 66128-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66128-1-Ig (OXA1L antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of HEK-293 using 66128-1-Ig

  WB result of OXA1L antibody (66128-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXA1L transfected HEK-293 cells.

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  WB analysis of NIH/3T3 using 66128-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66128-1-Ig (OXA1L antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 66128-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain slide using 66128-1-Ig (OXA1L Antibody) at dilution of 1:50.

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  IHC staining of human brain using 66128-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain slide using 66128-1-Ig (OXA1L Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: T-47D cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, HepG2 cells, L02 cells, Jurkat cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

66128-1-Ig targets OXA1L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: OXA1L fusion protein Ag13819
• Full Name: oxidase (cytochrome c) assembly 1-like
• Calculated Molecular Weight: 437 aa, 49 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC001669
• Gene Symbol: OXA1L
• Gene ID (NCBI): 5018
• RRID: AB_2881527
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OXA1L/Oxa1 is a member of the conserved Oxa1/YidC/Alb3 protein family and is required for cytochrome c oxidase assembly. The OXA1L protein was initially identified in yeast proteins involved in the biogenesis of the mitochondrial respiratory chain complexes. It locates in the inner membrane of mitochondria and may be involved in mediating the insertion of proteins from the matrix into the inner membrane.

Publications

Species	Application	Title
human	WB	Cell; An in vitro system to silence mitochondrial gene expression.; Authors - Luis Daniel Cruz-Zaragoza
human	WB	Cell; Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.; Authors - Ricarda Richter-Dennerlein
human	WB	Cell Rep; EMRE Is a Matrix Ca(2+) Sensor that Governs Gatekeeping of the Mitochondrial Ca(2+) Uniporter.; Authors - Horia Vais
mouse	WB	Cell Rep; Mitochondrial microproteins link metabolic cues to respiratory chain biogenesis; Authors - Chao Liang