OXR1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86359-3-RR (same clone as 86359-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86359-3-RR (OXR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86359-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 86359-3-RR (same clone as 86359-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86359-3-RR (OXR1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86359-3-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 86359-3-RR (same clone as 86359-3-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 86359-3-RR (OXR1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86359-3-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 86359-3-RR (same clone as 86359-3-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 86359-3-RR (OXR1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86359-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 86359-3-RR (same clone as 86359-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86359-3-RR (OXR1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86359-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86359-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86359-3-RR against Human OXR1 were performed. The affinity constant is 0.533 nM.

OXR1 Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 86359-3-PBS

Clone No.251027B10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

CHEGDD, Nbla00307, TLDC3

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Product Information

86359-3-PBS targets OXR1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4439

  Product name: Recombinant human OXR1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-351 aa of BC032710

  Sequence: MSFQKPKGTIEYTVESRDSLNSIALKFDTTPNELVQLNKLFSRAVVTGQVLYVPDPEYVSSVESSPSLSPVSPLSPTSSEAEFDKTTNPDVHPTEATPSSTFTGIRPARVVSSTSEEEEAFTEKFLKINCKYITSGKGTVSGVLLVTPNNIMFDPHKNDPLVQENGCEEYGIMCPMEEVMSAAMYKEILDSKIKESLPIDIDQLSGRDFCHSKKMTGSNTEEIDSRIRDAGNDSASTAPRSTEESLSEDVFTESELSPIREELVSSDELRQDKSSGASSESVQTVNQAEVESLTVKSESTGTPGHLRSDTEHSTNEVGTLCHKTDLNNLEMAIKEDQIADNFQGISGPKED

• Full Name: oxidation resistance 1
• Calculated Molecular Weight: 758 aa, 85 kDa
• Observed Molecular Weight: 120-140 kDa
• GenBank Accession Number: BC032710
• Gene Symbol: OXR1
• Gene ID (NCBI): 55074
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N573
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Oxidation resistance protein 1 (OXR1) belongs to the OXR1 family. The major function of OXR1 is to control the expression of genes that alleviate oxidative stress by increasing cellular resistance to reactive oxygen species (ROS) and the stress these molecules cause the cell. OXR1's ability to reduce oxidative stress and neurodegeneration in multiple diseases strongly suggests that it can be an effective therapeutic target (PMID: 33384581). Biochemical experiments show that OXR1 inhibits V1-ATPase and causes disassembly of the holoenzyme, suggesting that OXR1 plays a direct role in V-ATPase regulation (PMID: 34918374). OXR1 has 8 isoforms with the molecular mass of 25, 28, 34, 56, 94, 95 and 98 kDa. Sometimes higher molecular weight around 120-140 kDa can also be observed, which may be a modified variant of OXR1.