OXSR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

OXSR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 15611-1-AP

Synonyms

KIAA1101, OSR1, oxidative stress responsive 1, OXSR1

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Validation Data Gallery

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  WB analysis using 15611-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15611-1-AP (OXSR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15611-1-AP

  WB result of OXSR1 antibody (15611-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXSR1 transfected HeLa cells.

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  WB analysis of human liver using 15611-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15611-1-AP (OXSR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15611-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15611-1-AP (OXSR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15611-1-AP

  IP result of anti-OXSR1 (IP:15611-1-AP, 4ug; Detection:15611-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IP experiment of HEK-293 using 15611-1-AP

  IP result of anti-OXSR1 (IP:15611-1-AP, 4ug; Detection:15611-1-AP 1:300) with HEK-293 cells lysate 2320 ug.

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  IHC staining of human prostate cancer using 15611-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15611-1-AP (OXSR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15611-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15611-1-AP (OXSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 15611-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using OXSR1 antibody (15611-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human liver tissue, human testis tissue, HeLa cells, Jurkat cells, U-251 cells
• Positive IP detected in: HeLa cells, HEK-293 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

15611-1-AP targets OXSR1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OXSR1 fusion protein Ag8002
• Full Name: oxidative-stress responsive 1
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC008726
• Gene Symbol: OXSR1
• Gene ID (NCBI): 9943
• RRID: AB_2299030
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Oxidative-stress responsive 1(OXSR1) is also named as KIAA1101, OSR1 and belongs to the STE Ser/Thr protein kinase family. It contains an N-terminal Ste20-like ser/thr kinase domain and 2 C-terminal regions, which has a putative caspase-3 cleavage site at the end. OXSR1's interaction with WNK1 is required for NKCC function, and it modulates the G protein sensitivity of PAK by phosphorylation of PAK1.Western blot analysis detected Oxsr1 at an apparent molecular mass of 58 kD in all mouse tissues examined except thymus. Cell fractionation and immunofluorescence analysis of HeLa cells showed that OXSR1 was distributed throughout the cell and OXSR1 could phosphorylate a test substrate and itself(PMID:14707132).

Publications

Species	Application	Title
human	IHC,WB	Bioengineered; Upregulation of Oxidative stress-responsive 1(OXSR1) Predicts Poor Prognosis and Promotes Hepatocellular Carcinoma Progression.; Authors - Jianhui Chen
rat	WB,IF	Adv Sci (Weinh); Modulation of Cerebrospinal Fluid Dysregulation via a SPAK and OSR1 Targeted Framework Nucleic Acid in Hydrocephalus; Authors - Qiguang Wang
rat	WB	Exp Mol Pathol; Proteomic analysis of the effects of Dictyophora polysaccharide on arsenic-induced hepatotoxicity in rats; Authors - Xi Yan
rat	WB	Heliyon; Sevoflurane-induced regulation of NKCC1/KCC2 phosphorylation through activation of Spak/OSR1 kinase and cognitive impairment in ischemia-reperfusion injury in rats; Authors - Yuefeng Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jennifer (Verified Customer) (03-23-2022)

Image provided from optimisation plate for OXSR1 for rat brain lysates. Analysed using Simple Western Analysis (WES) by Protein Simple. Finalised antibody and protein concentration for samples produced a single band with no non-specific binding.

• Applications: Other
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Rat and mouse brain lysate

OXSR1 Antibody Other validation (1:100 dilution) in Rat and mouse brain lysate (Cat no:15611-1-AP)