OXT Polyclonal antibody

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  IP experiment of mouse pituitary gland using 18041-1-AP

  IP result of anti-OXT (IP:18041-1-AP, 4ug; Detection:18041-1-AP 1:300) with mouse pituitary gland tissue lysate 1440 ug.

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  IHC staining of human pituitary using 18041-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pituitary using 18041-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18041-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 18041-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 18041-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

OXT Polyclonal Antibody for IHC, IP, ELISA

Cat No. 18041-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF, IP, ELISA

Synonyms

Neurophysin 1, Ocytocin Neurophysin 1, Oxytocin, Oxytocin neurophysin 1, Oxytocin-neurophysin 1

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Tested Applications

• Positive IP detected in: mouse pituitary gland tissue
• Positive IHC detected in: human pituitary tissue, mouse ovary tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

18041-1-AP targets OXT in IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12672

  Product name: Recombinant human OXT protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 19-125 aa of BC101841

  Sequence: ACYIQNCPLGGKRAAPDLDVRKCLPCGPGGKGRCFGPNICCAEELGCFVGTAEALRCQEENYLPSPCQSGQKACGSGGRCAVLGLCCSPDGCHADPACDAEATFSQR

• Full Name: oxytocin, prepropeptide
• Calculated Molecular Weight: 125 aa, 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC101841
• Gene Symbol: OXT
• Gene ID (NCBI): 5020
• RRID: AB_2918056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P01178
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Oxytocin (OXT) is a neuropeptide involved in social-approach behaviors in humans and others mammals (PMID: 27325757). OXT is located on chromosome 20p13 and codes for a precursor protein that is synthetized to produce oxytocin and neurophysin I, and neurophysin I is the carrier protein for oxytocin (PMID: 1486803, PMID: 18566739). Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. Acts by binding to oxytocin receptor (OXTR) (PMID: 18174156).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Front Endocrinol (Lausanne); Targeted deletion of the pancreatic β-cell oxytocin receptor and its effects on metabolic regulation and β-cell health; Authors - Armando J Mendez