OXTR Polyclonal antibody

• Featured Product
• KD/KO Validated

OXTR Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 23045-1-AP

Synonyms

Oxytocin receptor, OT-R, OT R

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Validation Data Gallery

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  WB analysis using 23045-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23045-1-AP (OXTR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 23045-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23045-1-AP (OXTR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 23045-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 23045-1-AP (Oxytocin Receptor antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23045-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23045-1-AP (Oxytocin Receptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 23045-1-AP

  IP result of anti-OXTR (IP:23045-1-AP, 4ug; Detection:23045-1-AP 1:1000) with L02 cells lysate 1800ug.

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  IHC staining of human hysteromyoma using 23045-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23045-1-AP (OXTR antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human hysteromyoma using 23045-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23045-1-AP (OXTR antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 23045-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 23045-1-AP (Oxytocin Receptor antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 23045-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 23045-1-AP (Oxytocin Receptor antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, L02 cells
• Positive IP detected in: L02 cells
• Positive IHC detected in: human hysteromyoma tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 9 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Product Information

23045-1-AP targets OXTR in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OXTR fusion protein Ag19074
• Full Name: OXTR
• Calculated Molecular Weight: 389 aa, 43 kDa
• Observed Molecular Weight: 46 kDa, 67 kDa
• GenBank Accession Number: BC137443
• Gene Symbol: OXTR
• Gene ID (NCBI): 5021
• RRID: AB_2827425
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Oxytocin (OXT) is a neurohypophysial hormone that plays a role in lactation and parturition and in the central nervous system as a neurotransmitter involved in sex and maternal behavior (PMID: 1852313; 10811917). OXT acts through its receptor, OXTR, which belongs to the G protein-coupled 7-transmembrane receptor family. OXTR is expressed in the endometrium, myometrium, and mammary gland (PMID: 1313946). This antibody is raised against the C-terminal region of human OXTR. Two immunoreactive OXTR bands were found in the fat tissue, an unglycosylated 43 kDa form and a mature glycosylated 67 kDa form (PMID:32819381).

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun; PMID: 24915299; Authors - Christian Elabd
mouse	IF	Acta Physiol (Oxf); Oxytocin modulates neural processing of mitral/tufted cells in the olfactory bulb.; Authors - Changcheng Sun
mouse	IF	Oxid Med Cell Longev; PMID: 32082478; Authors - Daniela C Wigger
mouse	IHC	Antioxidants (Basel); ΔMST and the Regulation of Cardiac CSE and OTR Expression in Trauma and Hemorrhage.; Authors - Britta Trautwein
human	WB	Biochem Pharmacol; Synergistic antitumor activity of 5-fluorouracil and atosiban against microsatellite stable colorectal cancer through restoring GATA3.; Authors - Mengmeng Wang ; KD Validated
human	WB	Front Pharmacol; The Role of Formyl Peptide Receptor 1 in Uterine Contraction During Parturition.; Authors - Chaolu Chen