Osteopontin Polyclonal antibody

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  WB analysis using 30200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30200-1-AP (Osteopontin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 30200-1-AP

  WB result of Osteopontin antibody (30200-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Osteopontin transfected RAW 264.7 cells.

• 

  WB analysis using 30200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30200-1-AP (Osteopontin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30200-1-AP (Osteopontin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse stomach using 30200-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using Osteopontin antibody (30200-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of RAW 264.7 using 30200-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Osteopontin antibody (30200-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of RAW 264.7 using 30200-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using Osteopontin antibody (30200-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

Osteopontin Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

Cat No. 30200-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

OPN, 2AR, Bone sialoprotein 1, BSPI, Eta-1

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, rat kidney tissue, human kidney tissue, LPS and Brefeldin A treated RAW 264.7
• Positive IF-P detected in: mouse stomach tissue
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

30200-1-AP targets Osteopontin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg0317

  Product name: Recombinant Mouse Osteopontin protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 17-294 aa of NM_001204233.1

  Sequence: LPVKVTDSGSSEEKLYSLHPDPIATWLVPDPSQKQNLLAPQNAVSSEEKDDFKQETLPSNSNESHDHMDDDDDDDDDDGDHAESEDSVDSDESDESHHSDESDETVTASTQADTFTPIVPTVDVPNGRGDSLAYGLRSKSRSFQVSDEQYPDATDEDLTSHMKSGESKESLDVIPVAQLLSMPSDQDNNGKGSHESSQLDEPSLETHRLEHSKESQESADQSDVIDSQASSKASLEHQSHKFHSHKDKLVLDPKSKEDDRYLKFRISHELESSSSEVN

• Full Name: secreted phosphoprotein 1
• Calculated Molecular Weight: 32kd
• Observed Molecular Weight: 32 kDa, 60-70 kDa
• GenBank Accession Number: NM_001204233.1
• Gene Symbol: Osteopontin
• Gene ID (NCBI): 20750
• RRID: AB_3086261
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P10923
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Osteopontin (OPN) is also named as 2AR, minopontin, bone sialoprotein 1, Spp1, Eta-1 and Op, and belongs to the osteopontin family. Osteopontin is major non-collagenous bone protein that binds tightly to hydroxyapatite. It is a highly phosphorylated glycophosphoprotein having acidic characteristics and rich in aspartic acid (PMID: 30003880). And it acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (PMID:2351930, PMID:10657301). OPN, a multifunctional protein, has important functions on cardiovascular diseases, cancer, diabetes and kidney stone diseases and in the process of inflammation, biomineralization, cell viability and wound healing (PMID: 30003880). Osteopontin (OPN) is a pleiotropic glycoprotein expressed in various cell types in animals and in humans, including bone, immune, smooth muscle, epithelial and endothelial cells. Moreover, OPN is found in kidneys (in the thick ascending limbs of the loop of Henle and in distal nephrons) and urine (PMID:30456594). Osteopontin (OPN) is an acidic 60-70 kDa glycoprotein that is cleaved by proteases to yield 45-kDa and 24-kDa fragments (PMID: 10491621, PMID: 1995615)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Int J Biol Macromol; Cranial repair-promoting effect of oxidised sodium alginate/amino gelatine injectable hydrogel loaded with deer antler blood peptides; Authors - Taojing Yu
rat	WB	J Funct Biomater; Cu-MOF-Decorated 3D-Printed Scaffolds for Infection Control and Bone Regeneration; Authors - Ting Zhu
rat	WB,IHC	FASEB J; Mitochondrial Calcium Uniporter-Mediated Regulation of the SIRT3/GSK3β/β-Catenin Signaling Pathway in Vascular Remodeling; Authors - Min Jiang
rat	IF	Acta Biomater; Deciphering the Heterogeneity of Calcified Structure in Different Entheses via High-resolution Characterization Techniques; Authors - Ziyang Lin
mouse	WB	Front Pharmacol; An integrative pharmacology-based study on the pharmacological activity and mechanism of xiaoji-chenpi formula (XCF) against MAFLD; Authors - Shufei Liang
mouse	IHC	Osteoarthritis Cartilage; M2 macrophages regulate nucleus pulposus cell extracellular matrix synthesis through the OPN-CD44 axis in intervertebral disc degeneration; Authors - Zhiwen Tao