Validation Data Gallery
|Positive WB detected in||Jurkat cells, HEK-293 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11815-1-AP targets NOLC1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NOLC1 fusion protein Ag2382|
|Full Name||nucleolar and coiled-body phosphoprotein 1|
|Calculated molecular weight||130 kDa|
|Observed molecular weight||130 kDa|
|GenBank accession number||BC006769|
|Gene ID (NCBI)||9221|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Nucleolar and coiled-body phosphoprotein 1 (NOLC1) is a phosphoprotein that transiently associates with the mature nucleolar H/ACA and C/D box small nucleolar ribonucleoproteins (snoRNPs), guiding site-specific 2'-O-meth-ylation and pseudouridylation of pre-rRNAs [PMID:21266110]. It contains a nuclear localization signal binding sequence and is thought to shuttle between the nucleolus and the cytoplasm. NOLC1 plays an essential role in the synthesis of rRNA and the biosynthesis of ribosomes [PMID:8972203].
|Product Specific Protocols|
|WB protocol for NOLC1 antibody 11815-1-AP||Download protocol|
|IHC protocol for NOLC1 antibody 11815-1-AP||Download protocol|
|IF protocol for NOLC1 antibody 11815-1-AP||Download protocol|
|IP protocol for NOLC1 antibody 11815-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases
Cell-fate determination by ubiquitin-dependent regulation of translation.
Multisite dependency of an E3 ligase controls monoubiquitylation-dependent cell fate decisions.
Acta Pharmacol Sin
Atypical chemokine receptor 3 induces colorectal tumorigenesis in mice by promoting β-arrestin-NOLC1-fibrillarin-dependent rRNA biogenesis.
J Cell Sci
Novel stress granules-like structures are induced via a paracrine mechanism during viral infection.
Ribosome changes reprogram translation for chemosurvival in G0 leukemic cells