NOLC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NOLC1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 11815-1-AP

Synonyms

KIAA0035, NOLC1, NOPP130, NOPP140, NS5ATP13, Nucleolar 130 kDa protein, Nucleolar phosphoprotein p130

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Validation Data Gallery

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  WB analysis of Jurkat using 11815-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11815-1-AP (NOLC1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11815-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11815-1-AP (NOLC1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11815-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11815-1-AP (NOLC1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11815-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11815-1-AP (NOLC1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11815-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11815-1-AP (NOLC1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11815-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11815-1-AP (NOLC1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11815-1-AP

  IP result of anti-NOLC1 (IP:11815-1-AP, 4ug; Detection:11815-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human colon cancer using 11815-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11815-1-AP (NOLC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11815-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11815-1-AP (NOLC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11815-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11815-1-AP (NOLC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11815-1-AP

  Immunofluorescent analysis of HepG2 cells, using NOLC1 antibody 11815-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

11815-1-AP targets NOLC1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOLC1 fusion protein Ag2382
• Full Name: nucleolar and coiled-body phosphoprotein 1
• Calculated Molecular Weight: 130 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC006769
• Gene Symbol: NOLC1
• Gene ID (NCBI): 9221
• RRID: AB_2235983
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nucleolar and coiled-body phosphoprotein 1 (NOLC1) is a phosphoprotein that transiently associates with the mature nucleolar H/ACA and C/D box small nucleolar ribonucleoproteins (snoRNPs), guiding site-specific 2'-O-meth-ylation and pseudouridylation of pre-rRNAs [PMID:21266110]. It contains a nuclear localization signal binding sequence and is thought to shuttle between the nucleolus and the cytoplasm. NOLC1 plays an essential role in the synthesis of rRNA and the biosynthesis of ribosomes [PMID:8972203].

Publications

Species	Application	Title
human	IF	Nat Biotechnol; Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases; Authors - Matthew T N Yarnall
human	WB	Nature; Cell-fate determination by ubiquitin-dependent regulation of translation.; Authors - Achim Werner
human	WB,IF	Elife; Multisite dependency of an E3 ligase controls monoubiquitylation-dependent cell fate decisions.; Authors - Achim Werner ; KD Validated
human,mouse	WB,IHC	Acta Pharmacol Sin; Atypical chemokine receptor 3 induces colorectal tumorigenesis in mice by promoting β-arrestin-NOLC1-fibrillarin-dependent rRNA biogenesis.; Authors - Juan Yang
	WB,IF	J Cell Sci; Novel stress granules-like structures are induced via a paracrine mechanism during viral infection.; Authors - Valentina Iadevaia
human	WB	Sci Adv; Ribosome changes reprogram translation for chemosurvival in G0 leukemic cells; Authors - Chandreyee Datta