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  • KD/KO Validated

NOLC1 Polyclonal antibody

NOLC1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11815-1-AP

Synonyms

KIAA0035, NOLC1, NOPP130, NOPP140, NS5ATP13, Nucleolar 130 kDa protein, Nucleolar phosphoprotein p130



Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11815-1-AP targets NOLC1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOLC1 fusion protein Ag2382
Full Name nucleolar and coiled-body phosphoprotein 1
Calculated molecular weight 130 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC006769
Gene symbol NOLC1
Gene ID (NCBI) 9221
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nucleolar and coiled-body phosphoprotein 1 (NOLC1) is a phosphoprotein that transiently associates with the mature nucleolar H/ACA and C/D box small nucleolar ribonucleoproteins (snoRNPs), guiding site-specific 2'-O-meth-ylation and pseudouridylation of pre-rRNAs [PMID:21266110]. It contains a nuclear localization signal binding sequence and is thought to shuttle between the nucleolus and the cytoplasm. NOLC1 plays an essential role in the synthesis of rRNA and the biosynthesis of ribosomes [PMID:8972203].

Protocols

Product Specific Protocols
WB protocol for NOLC1 antibody 11815-1-APDownload protocol
IHC protocol for NOLC1 antibody 11815-1-APDownload protocol
IF protocol for NOLC1 antibody 11815-1-APDownload protocol
IP protocol for NOLC1 antibody 11815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Elife

Multisite dependency of an E3 ligase controls monoubiquitylation-dependent cell fate decisions.

Authors - Achim Werner
  • KD Validated
humanWB

Nature

Cell-fate determination by ubiquitin-dependent regulation of translation.

Authors - Achim Werner