P2RX3 Polyclonal antibody

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• P2RX3 Polyclonal antibody (17843-1-AP)

P2RX3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17843-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, ELISA

Synonyms

ATP receptor, P2X purinoceptor 3, P2X3, Purinergic receptor

• 

  WB analysis of Jurkat using 17843-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17843-1-AP (P2RX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 17843-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17843-1-AP (P2RX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 17843-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17843-1-AP (P2RX3 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 17843-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17843-1-AP (P2RX3 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17843-1-AP targets P2RX3 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12207

  Product name: Recombinant human P2RX3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 44-326 aa of BC074792

  Sequence: LHEKAYQVRDTAIESSVVTKVKGSGLYANRVMDVSDYVTPPQGTSVFVIITKMIVTENQMQGFCPESEEKYRCVSDSQCGPERLPGGGILTGRCVNYSSVLRTCEIQGWCPTEVDTVETPIMMEAENFTIFIKNSIRFPLFNFEKGNLLPNLTARDMKTCRFHPDKDPFCPILRVGDVVKFAGQDFAKLARTGGVLGIKIGWVCDLDKAWDQCIPKYSFTRLDSVSEKSSVSPGYNFRFAKYYKMENGSEYRTLLKAFGIRFDVLVYGNAGKFNIIPTIISSV

• Full Name: purinergic receptor P2X, ligand-gated ion channel, 3
• Calculated Molecular Weight: 397 aa, 44 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC074792
• Gene Symbol: P2RX3
• Gene ID (NCBI): 5024
• RRID: AB_2878449
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P56373
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The anti-P2X3 Receptor antibody (Cat# 17843-1-AP) has 8 total citations published in journals including J Ethnopharmacol, Int Immunopharmacol, Biomolecules, Am J Physiol Cell Physiol, Neuropharmacology, J Clin Med, Sleep Breath, and Transl Androl Urol. Research fields span inflammatory pain, purinergic receptor signaling, cough hypersensitivity, hippocampal neuronal regulation, allergic contact dermatitis, ureteropelvic junction obstruction, chronic intermittent hypoxia-induced enuresis, and overactive bladder.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	J Ethnopharmacol; The ethanol extract of Scutellaria baicalensis Georgi attenuates complete Freund's adjuvant (CFA)-induced inflammatory pain by suppression of P2X3 receptor; Authors - Li Gao
pig	IHC,IF	Biomolecules; Activation of Adenosine Triphosphate-Gated Purinergic 2 Receptor Channels by Transient Receptor Potential Vanilloid Subtype 4 in Cough Hypersensitivity; Authors - Wanzhen Li
rat	WB	Int Immunopharmacol; 4-Methylesculetin attenuates inflammatory pain via inhibition of Sp1-TRPV1 and inflammation related signaling pathways; Authors - Weixin Zhao
mouse	WB	Am J Physiol Cell Physiol; Regulatory function of praja ring finger ubiquitin ligase 2 mediated by the P2rx3/P2rx7 axis in mouse hippocampal neuronal cells.; Authors - Mengting Gong
mouse	IF	Neuropharmacology; Study on the effect and mechanism of sesamin on P2X3-related cheek allergic contact dermatitis in mice induced by SADBE stimulation.; Authors - Xiaoqian Ma
human	IHC	J Clin Med; Interstitial Cells of Cajal and P2X3 Receptors at Ureteropelvic Junction Obstruction and Their Relationship with Pain Response; Authors - Dominika Borselle