P2RX3 Polyclonal antibody

P2RX3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17843-1-AP

Synonyms

ATP receptor, P2RX3, P2X purinoceptor 3, P2X3, Purinergic receptor



Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17843-1-AP is P2RX3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P2RX3 fusion protein Ag12207
Full Name purinergic receptor P2X, ligand-gated ion channel, 3
Calculated molecular weight 397 aa, 44 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC074792
Gene symbol P2RX3
Gene ID (NCBI) 5024
RRIDAB_2878449
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for P2RX3 antibody 17843-1-APDownload protocol
IHC protocol for P2RX3 antibody 17843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Ethnopharmacol

The ethanol extract of Scutellaria baicalensis Georgi attenuates complete Freund's adjuvant (CFA)-induced inflammatory pain by suppression of P2X3 receptor

Authors - Li Gao
mouseWB

Am J Physiol Cell Physiol

Regulatory function of praja ring finger ubiquitin ligase 2 mediated by the P2rx3/P2rx7 axis in mouse hippocampal neuronal cells.

Authors - Mengting Gong
ratWB,IHC

Sleep Breath

Therapeutic effects and modulatory mechanism of Alpiniae oxyphyllae Fructus in chronic intermittent hypoxia induced enuresis in rats.

Authors - Miao-Shang Su