P2RX5 Polyclonal antibody

P2RX5 Polyclonal Antibody for FC, IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 19012-1-AP

Synonyms

ATP receptor, P2RX5, P2X purinoceptor 5, P2X5, P2X5R, Purinergic receptor



Tested Applications

Positive WB detected inBxPC-3 cells, HL-60 cells, MDA-MB-453s cells
Positive IF detected inBxPC-3 cells
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 1 publications below

Product Information

19012-1-AP targets P2RX5 in WB, IF, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P2RX5 fusion protein Ag5297
Full Name purinergic receptor P2X, ligand-gated ion channel, 5
Calculated Molecular Weight 422 aa, 47 kDa
Observed Molecular Weight 44 kDa, 49 kDa, 116 kDa
GenBank Accession NumberBC039015
Gene Symbol P2RX5
Gene ID (NCBI) 5026
RRIDAB_2299079
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for P2RX5 antibody 19012-1-APDownload protocol
IF protocol for P2RX5 antibody 19012-1-APDownload protocol
FC protocol for P2RX5 antibody 19012-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

PLoS One

A Truncation Variant of the Cation Channel P2RX5 Is Upregulated during T Cell Activation.

Authors - Pierre Abramowski