P2RY2 Polyclonal antibody

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• P2RY2 Polyclonal antibody (20297-1-AP)

P2RY2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20297-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

ATP receptor, HP2U, P2RU1, P2U, P2U purinoceptor 1

• 

  WB analysis using 20297-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20297-1-AP (P2RY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 20297-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20297-1-AP (P2RY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 20297-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20297-1-AP (P2RY2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HL-60 cells, MCF-7 cells, PC-3 cells, MDA-MB-231 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20297-1-AP targets P2RY2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14107

  Product name: Recombinant human P2RY2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 268-377 aa of BC012104

  Sequence: YYSFRSLDLSCHTLNAINMAYKVTRPLASANSCLDPVLYFLAGQSLVRFARDAKPPTGPSPATPARRRLGLRRSDRTDMQRIEDVLGSSEDSRRTESTPAGSENTKDIRL

• Full Name: purinergic receptor P2Y, G-protein coupled, 2
• Calculated Molecular Weight: 377 aa, 42 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC012104
• Gene Symbol: P2RY2
• Gene ID (NCBI): 5029
• RRID: AB_3669332
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P41231
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P2RY2, also named P2RU1, P2U1, and P2Y2, belongs to the G-protein coupled receptor 1 family. It is a receptor for ATP and UTP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system. Extracellular ATP then acts on purinergic receptors, including P2RY2, to activate proline-rich tyrosine kinase 2 (Pyk2) kinase and transient plasma membrane depolarization.

Publications

What published studies show

The Anti-P2RY2 polyclonal antibody (Cat# 20297-1-AP) has 2 total citations. These appear in International Journal of Biological Macromolecules (IF 8.7) and Tissue and Cell (IF 3.1). Both studies applied the antibody for Western blot in human samples, investigating P2RY2's role in esophageal squamous cell carcinoma progression and as a predictive biomarker in gastric cancer, respectively. The research fields span oncology and molecular biology.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Int J Biol Macromol; Upregulation of has_circ_0008389 promotes esophageal squamous cell carcinoma progression via miR-761 sponging and P2RY2 mRNA stabilization.; Authors - Zherui Shao
human	WB	Tissue Cell; P2Y2 receptor as a favorable predictor of gastric cancer.; Authors - Yu-Qing Wu