P2RY2 Polyclonal antibody

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  WB analysis using 20297-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20297-1-AP (P2RY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20297-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20297-1-AP (P2RY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 20297-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20297-1-AP (P2RY2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

P2RY2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20297-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

ATP receptor, HP2U, P2RU1, P2U, P2U purinoceptor 1

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Tested Applications

• Positive WB detected in: HL-60 cells, MCF-7 cells, PC-3 cells, MDA-MB-231 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20297-1-AP targets P2RY2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14107

  Product name: Recombinant human P2RY2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 268-377 aa of BC012104

  Sequence: YYSFRSLDLSCHTLNAINMAYKVTRPLASANSCLDPVLYFLAGQSLVRFARDAKPPTGPSPATPARRRLGLRRSDRTDMQRIEDVLGSSEDSRRTESTPAGSENTKDIRL

• Full Name: purinergic receptor P2Y, G-protein coupled, 2
• Calculated Molecular Weight: 377 aa, 42 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC012104
• Gene Symbol: P2RY2
• Gene ID (NCBI): 5029
• RRID: AB_3669332
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P41231
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P2RY2, also named P2RU1, P2U1, and P2Y2, belongs to the G-protein coupled receptor 1 family. It is a receptor for ATP and UTP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system. Extracellular ATP then acts on purinergic receptors, including P2RY2, to activate proline-rich tyrosine kinase 2 (Pyk2) kinase and transient plasma membrane depolarization.