P3H3 Recombinant monoclonal antibody

• 

  WB analysis using 86813-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86813-1-RR (LEPREL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

P3H3 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86813-1-RR

Clone No.251845H1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

LEPREL2, EC:1.14.11.7, GRCB, HSU47926, leprecan like 2

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Tested Applications

• Positive WB detected in: MDA-MB-453 cells, NIH/3T3 cells, mouse ovary tissue, rat ovary tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86813-1-RR targets P3H3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag8850

  Product name: Recombinant human LEPREL2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 223-605 aa of BC017217

  Sequence: RALNQYQAQLGEPRPGLGPREDIQRFILRSLGEKRQLYYAMEHLGTSFKDPDPWTPAALIPEALREKLREDQEKRPWDHEPVKPKPLTYWKDVLLLEGVTLTQDSRQLNGSERAVLDGLLTPAECGVLLQLAKDAAGAGARSGYRGRRSPHTPHERFEGLTVLKAAQLARAGTVGSQGAKLLLEVSERVRTLTQAYFSPERPLHLSFTHLVCRSAIEGEQEQRMDLSHPVHADNCVLDPDTGECWREPPAYTYRDYSGLLYLNDDFQGGDLFFTEPNALTVTARVRPRCGRLVAFSSGVENPHGVWAVTRGRRCALALWHTWAPEHREQEWIEAKELLQESQEEEEEEEEEMPSKDPSPEPPSRRHQRVQDKTGRAPRVREEL

• Full Name: leprecan-like 2
• Calculated Molecular Weight: 736 aa, 82 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC017217
• Gene Symbol: LEPREL2
• Gene ID (NCBI): 10536
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8IVL6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P3H3 (prolyl 3-hydroxylase 3, also known as LEPREL2) is a member of the leprecan family of proteins, which also include P3H1, P3H2, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H3 is thought to reside in the endoplasmic reticulum and has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V.