Tested Applications
| Positive WB detected in | A431 cells, HT-1080 cells, BGC-823 cells, PC-3 cells |
| Positive IP detected in | LNCaP cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12658-1-AP targets P4HA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3292 Product name: Recombinant human P4HA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 238-534 aa of BC034998 Sequence: HQRANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQKYEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE Predict reactive species |
| Full Name | prolyl 4-hydroxylase, alpha polypeptide I |
| Calculated Molecular Weight | 534 aa, 61 kDa |
| Observed Molecular Weight | 61 kDa |
| GenBank Accession Number | BC034998 |
| Gene Symbol | P4HA1 |
| Gene ID (NCBI) | 5033 |
| RRID | AB_2283162 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13674 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
P4HA1 (Prolyl 4-hydroxylase subunit alpha-1) is also known as P4HA and belongs to the P4HA family, which plays a central role in collagen synthesis. P4HA1 catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. The gene encodes a polypeptide of 517 amino acid residues and a signal peptide of 17 amino acids and the full length protein has two glycosylation sites (uniprot). It can exist as a heteromer, dimer or tetramer (refer to: GENATLAS). P4HA1 has some isoforms with the MW 61-63 kDa and 57-59 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for P4HA1 antibody 12658-1-AP | Download protocol |
| IHC protocol for P4HA1 antibody 12658-1-AP | Download protocol |
| IP protocol for P4HA1 antibody 12658-1-AP | Download protocol |
| WB protocol for P4HA1 antibody 12658-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The P4HA1 Polyclonal antibody (Cat# 12658-1-AP) has accumulated 37 citations in journals including Nature, Cancer Cell, Cell Death & Differentiation, Journal of Experimental Medicine, Leukemia, Acta Pharm Sin B, Cell Signaling, and Molecular Oncology. Associated research fields encompass oncology (prostate, lung, melanoma, glioblastoma, gastric, esophageal, colorectal, and ovarian cancers), myocardial fibrosis, angiogenesis, collagen metabolism, ferroptosis, glycolysis, immune regulation, and connective tissue disorders. Primary applications include Western blot and IHC.
| Species | Application | Title |
|---|---|---|
Cancer Cell Targeting P4HA1 promotes CD8+ T cell progenitor expansion toward immune memory and systemic anti-tumor immunity | ||
Redox Biol Targeting HIF-1α promotes ferroptosis and boosts antitumor immunity in MSS colorectal cancer. | ||
Acta Pharm Sin B Palmitoylated SARM1 targeting P4HA1 promotes collagen deposition and myocardial fibrosis: A new target for anti-myocardial fibrosis. | ||
Cell Death Differ Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation | ||
J Exp Med IL-17-induced HIF1α drives resistance to anti-PD-L1 via fibroblast-mediated immune exclusion. |
