P4HA1 Monoclonal antibody, PBS Only

• 

  WB analysis using 66101-1-Ig (same clone as 66101-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 66101-1-Ig (same clone as 66101-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66101-1-Ig (same clone as 66101-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 66101-1-Ig (same clone as 66101-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

• 

  WB analysis of ROS1728 using 66101-1-Ig (same clone as 66101-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Neuro-2a using 66101-1-Ig (same clone as 66101-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

• 

  WB analysis of 4T1 using 66101-1-Ig (same clone as 66101-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 66101-1-Ig (same clone as 66101-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66101-1-Ig (same clone as 66101-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66101-1-Ig (same clone as 66101-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66101-1-Ig (same clone as 66101-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66101-1-Ig (same clone as 66101-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66101-1-Ig (same clone as 66101-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

P4HA1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66101-1-PBS

Clone No.1H10A6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

1H10A6, EC:1.14.11.2, P4HA, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, Prolyl 4-hydroxylase subunit alpha-1

---

Product Information

66101-1-PBS targets P4HA1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17867

  Product name: Recombinant human P4HA1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 236-534 aa of BC034998

  Sequence: PEHQRANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQKYEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE

• Full Name: prolyl 4-hydroxylase, alpha polypeptide I
• Calculated Molecular Weight: 534 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC034998
• Gene Symbol: P4HA1
• Gene ID (NCBI): 5033
• RRID: AB_2881500
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13674
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

P4HA1(Prolyl 4-hydroxylase subunit alpha-1) is also named as P4HA and belongs to the P4HA family , which play a central role in collagen synthesis. P4HA1 catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. The gene encodes a polypeptide of 517 amino acid residues and a signal peptide of 17 amino acids and the full length protein has two glycosylation sites (uniprot). It can exsit as a heteromer, dimer or tetramer (refer to: GENATLAS). P4HA1 has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 61 kDa and 59 kDa. This antibody is specific to P4HA1.