P4HA2 Polyclonal antibody

• Featured Product
• KD/KO Validated

P4HA2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

21

Cat no : 13759-1-AP

Synonyms

4 PH alpha 2, P4HA2

---

Validation Data Gallery

• 

  WB analysis of HeLa using 13759-1-AP

  WB result of P4HA2 antibody (13759-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P4HA2 transfected HeLa cells.

• 

  WB analysis using 13759-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13759-1-AP (P4HA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13759-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13759-1-AP (P4HA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13759-1-AP

  IP result of anti-P4HA2 (IP:13759-1-AP, 4ug; Detection:13759-1-AP 1:1000) with HeLa cells lysate 1200ug.

• 

  IHC staining of human cervical cancer using 13759-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13759-1-AP (P4HA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13759-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13759-1-AP (P4HA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary tissue using 13759-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 26424-1-AP( LHCGR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary tissue using 13759-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 26424-1-AP( LHCGR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 13759-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13759-1-AP (P4HA2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 13759-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13759-1-AP (P4HA2 antibody) at dilution of 1:50 (under 10x lens).

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human cervical cancer tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 16 publications below
• IHC: See 8 publications below
• IF: See 4 publications below

Product Information

13759-1-AP targets P4HA2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P4HA2 fusion protein Ag4714
• Full Name: prolyl 4-hydroxylase, alpha polypeptide II
• Calculated Molecular Weight: 533 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC035813
• Gene Symbol: P4HA2
• Gene ID (NCBI): 8974
• RRID: AB_2156286
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Prolyl 4-hydroxylase catalyzes the posttranslational formation of 4-hydroxyproline in collagens. The vertebrate enzyme is a tetramer of 2 alpha subunits and 2 beta subunits. P4HA2 is one of at least 2 alpha subunit isoforms. It catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. This pprotein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB,IHC,IF	Hepatology; P4HA2 induces hepatic ductular reaction and biliary fibrosis in chronic cholestatic liver diseases; Authors - Jun Zhang ; KD Validated
human	IHC	Blood; Prolyl 4-hydroxylase 2 promotes B-cell lymphoma progression via hydroxylation of Carabin.; Authors - Wei Jiang
		Nat Commun; Melanoblast transcriptome analysis reveals pathways promoting melanoma metastasis.; Authors - Kerrie L Marie
human	WB	EBioMedicine; Aspirin targets P4HA2 through inhibiting NF-κB and LMCD1-AS1/let-7g to inhibit tumour growth and collagen deposition in hepatocellular carcinoma.; Authors - Tianjiao Wang
human	WB	Cancer Res; Epigenetic SMAD3 repression in tumor-associated fibroblasts impairs fibrosis and response to the antifibrotic drug nintedanib in lung squamous cell carcinoma.; Authors - Rafael Ikemori
human	WB,IHC	Oncogene; Hepatitis B virus X protein promotes the development of liver fibrosis and hepatoma through downregulation of miR-30e targeting P4HA2 mRNA.; Authors - G X Feng