P53 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 80077-1-RR (same clone as 80077-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

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  WB analysis of zebrafish using 80077-1-RR (same clone as 80077-1-PBS)

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 80077-1-RR (same clone as 80077-1-PBS)

  WB result of P53 antibody (80077-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P53 transfected A431 cells. This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 80077-1-RR (same clone as 80077-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

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  WB analysis of SW480 using 80077-1-RR (same clone as 80077-1-PBS)

  SW480 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 80077-1-RR (same clone as 80077-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 80077-1-RR (same clone as 80077-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 80077-1-RR (same clone as 80077-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue slide using 80077-1-RR (p53 antibody) at dilution of 1:1000 (under 10x lens).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 7.0). Staining was performed on the Epredia 480S Autostainer.​ This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 80077-1-RR (same clone as 80077-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue slide using 80077-1-RR (p53 antibody) at dilution of 1:1000 (under 40x lens).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 7.0). Staining was performed on the Epredia 480S Autostainer. This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 80077-1-RR (same clone as 80077-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80077-1-RR (P53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 80077-1-RR (same clone as 80077-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using P53 antibody (80077-1-RR, Clone: 2H13 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 80077-1-RR (same clone as 80077-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (80077-1-RR, Clone: 2H13 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 80077-1-RR (same clone as 80077-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human P53 (80077-1-RR, Clone:2H13) (red) labeled with FlexAble CoraLite®488 Antibody Labeling Kit for Rabbit IgG (KFA001), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

P53 Uni-rAb^® Recombinant Antibody for WB, IHC-Autostainer, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 80077-1-PBS

Clone No.2H13

Host / Isotype

Rabbit / IgG

Reactivity

human, zebrafish

Applications

WB, IHC-Autostainer, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

TP53, 2H13, Antigen NY-CO-13, tumor protein p53, Tumor suppressor p53

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Product Information

80077-1-PBS targets P53 in WB, IHC-Autostainer, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, zebrafish samples.

• Tested Reactivity: human, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tumor protein p53
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC003596
• Gene Symbol: P53
• Gene ID (NCBI): 7157
• RRID: AB_2918862
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04637
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)