P5CS Monoclonal antibody, PBS Only

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  WB analysis using 68184-1-Ig (same clone as 68184-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68184-1-Ig (P5CS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68184-1-PBS in a different storage buffer formulation.

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  WB analysis of HAP1 using 68184-1-Ig (same clone as 68184-1-PBS)

  WB result of P5CS antibody (68184-1-Ig; 1:10000; room temperature for 1.5 hours) with wild-type and sg-P5CS transfected HAP1 cells. This data was developed using the same antibody clone with 68184-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse colon using 68184-1-Ig (same clone as 68184-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 68184-1-Ig (P5CS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68184-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat colon using 68184-1-Ig (same clone as 68184-1-PBS)

  rat colon tissue were subjected to SDS PAGE followed by western blot with 68184-1-Ig (P5CS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68184-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

P5CS Monoclonal Antibody for WB, ELISA

Cat No. 68184-1-PBS

Clone No.3B1E11

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ALDH18A1, 3B1E11, Aldehyde dehydrogenase family 18 member A1, Delta-1-pyrroline-5-carboxylate synthase, Gamma-glutamyl kinase

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Product Information

68184-1-PBS targets P5CS in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12100

  Product name: Recombinant human ALDH18A1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-362 aa of BC106054

  Sequence: MLSQVYRCGFQPFNQHLLPWVKCTTVFRSHCIQPSVIRHVRSWSNIPFITVPLSRTHGKSFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMLVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKEMAIPVLEARACAAAGQSGLMALYEAMFTQYSICAAQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIVNTNDAVVPPAEPNSDLQGVNVISVKDNDSLAARLAVEMKTDLLIVLSDVEGLFDSPPGSDDAKLIDIFYPGDQQSVTFGIKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKVSGHVITDIVEGKKVGTFFSEVKPAGPT

• Full Name: aldehyde dehydrogenase 18 family, member A1
• Calculated Molecular Weight: 795 aa, 87 kDa
• Observed Molecular Weight: 87 kDa
• GenBank Accession Number: BC106054
• Gene Symbol: P5CS
• Gene ID (NCBI): 5832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P54886
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Delta 1-pyrroline-5-carboxylate synthetase (P5CS), also named as GSAS, P5CS, PYCS, catalyzes the ATP and the NAD(P)H-dependent conversion of L-glutamate to glutamic gamma-semialdehyde (GSA) which is the metabolic precursor for proline biosynthesis(PMID:8761662). This protein includes 2 domains: glutamate 5-kinase and gamma-glutamyl phosphate reductase. In the N-terminal section, it belongs to the glutamate 5-kinase family and in the C-terminal section, it belongs to the gamma-glutamyl phosphate reductase family. Defects in ALDH18A1 are the cause of cutis laxa, autosomal recessive, type 3A (ARCL3A)(PMID:18478038). It has 2 isoforms produced by alternative splicing.