PI3 Kinase p85 Alpha Polyclonal antibody

• 

  WB analysis using 30092-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30092-1-AP (P85a; PIK3R1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30092-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30092-1-AP (P85a; PIK3R1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

P85a; PIK3R1 Polyclonal Antibody for WB, ELISA

Cat No. 30092-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IF, ELISA

Synonyms

GRB1, p85, p85 ALPHA, PI3 kinase subunit p85 alpha, PI3K regulatory subunit alpha, PIK3R1

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

30092-1-AP targets P85a; PIK3R1 in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC030815
• Gene Symbol: PI3 Kinase p85 Alpha
• Gene ID (NCBI): 5295
• RRID: AB_3086227
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P27986
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Acta Biochim Biophys Sin (Shanghai); HADHA promotes esophageal cancer progression by activating mTOR signaling and the SP1/MDM2 axis; Authors - Xusheng Ding
human	WB	J Ethnopharmacol; Cornus officinalis loganin attenuates acute lung injury in mice via regulating the PI3K/AKT/NLRP3 axis; Authors - Yiran Liu
human	WB,IF	Int J Biol Sci; BRD7 Inhibited Immune Escape in Nasopharyngeal Carcinoma via Inhibiting PD-L1 Expression; Authors - Yilin Guo
human	WB	Front Pharmacol; Immune-related gene risk model establishment and role of key gene FUCA1 in malignant pleural mesothelioma; Authors - Lin Shi
		Cell Death Dis; The dual mechanism of m6A demethylase ALKBH5 in regulating energy metabolism during exposure to MC-LR; Authors - Xiaoya Sun