PI3 Kinase p85 Alpha Polyclonal antibody

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• PI3 Kinase p85 Alpha Polyclonal antibody (30092-1-AP)

P85a; PIK3R1 Polyclonal Antibody for WB, ELISA

Cat No. 30092-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (3)

Applications

WB, IF, ELISA

Synonyms

GRB1, p85, p85 ALPHA, PI3 kinase subunit p85 alpha, PI3K regulatory subunit alpha, PIK3R1

• 

  WB analysis using 30092-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30092-1-AP (P85a; PIK3R1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 30092-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30092-1-AP (P85a; PIK3R1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30092-1-AP targets P85a; PIK3R1 in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC030815
• Gene Symbol: PI3 Kinase p85 Alpha
• Gene ID (NCBI): 5295
• RRID: AB_3086227
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P27986
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Bioorg Chem; MFG-E8-derived antioxidant peptides attenuate dexamethasone-induced skeletal muscle metabolic dysfunction via PI3K/Akt-GLUT4-PPARα signaling pathway.; Authors - He Li
human, mouse	WB	Biomater Adv; Rehmannia-derived nanovesicles hijack the cell cycle to suppress oral squamous cell carcinoma via PI3K-AKT pathway blockade.; Authors - Liyu Huang
human	WB	J Ethnopharmacol; Cornus officinalis loganin attenuates acute lung injury in mice via regulating the PI3K/AKT/NLRP3 axis; Authors - Yiran Liu
human	WB	Front Pharmacol; Immune-related gene risk model establishment and role of key gene FUCA1 in malignant pleural mesothelioma; Authors - Lin Shi
human	WB	Sci Rep; Elevated SLC4A11 expression promotes OV progression via interaction with EGFR; Authors - Qihua Peng
mouse	WB	Acta Biochim Biophys Sin (Shanghai); HADHA promotes esophageal cancer progression by activating mTOR signaling and the SP1/MDM2 axis; Authors - Xusheng Ding