Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30092-1-AP targets P85a; PIK3R1 in WB, IF, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | phosphoinositide-3-kinase, regulatory subunit 1 (alpha) |
| Calculated Molecular Weight | 85 kDa |
| Observed Molecular Weight | 85 kDa |
| GenBank Accession Number | BC030815 |
| Gene Symbol | PI3 Kinase p85 Alpha |
| Gene ID (NCBI) | 5295 |
| RRID | AB_3086227 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P27986 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for P85a; PIK3R1 antibody 30092-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PI3 Kinase p85 Alpha polyclonal antibody (Cat# 30092-1-AP) has 11 total citations spanning journals including Cell Death & Disease, International Journal of Biological Sciences, Journal of Ethnopharmacology, Biomaterials Advances, International Immunopharmacology, Frontiers in Pharmacology, Bioorganic Chemistry, Scientific Reports, Acta Biochimica et Biophysica Sinica, and Frontiers in Oncology. Research fields encompass cancer biology (lung, ovarian, breast, esophageal, nasopharyngeal carcinomas), PI3K/AKT signaling, metabolic regulation, and immune-related mechanisms.
| Species | Application | Title |
|---|---|---|
Cell Death Dis The dual mechanism of m6A demethylase ALKBH5 in regulating energy metabolism during exposure to MC-LR | ||
Int J Biol Sci BRD7 Inhibited Immune Escape in Nasopharyngeal Carcinoma via Inhibiting PD-L1 Expression | ||
J Ethnopharmacol Cornus officinalis loganin attenuates acute lung injury in mice via regulating the PI3K/AKT/NLRP3 axis | ||
Biomater Adv Rehmannia-derived nanovesicles hijack the cell cycle to suppress oral squamous cell carcinoma via PI3K-AKT pathway blockade. | ||
Int Immunopharmacol Vitamin D against diabetic adipose tissue inflammation through SHP-1/STAT3 pathway | ||
Front Pharmacol Immune-related gene risk model establishment and role of key gene FUCA1 in malignant pleural mesothelioma |



