PABPN1 Recombinant monoclonal antibody

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• PABPN1 Recombinant monoclonal antibody (87392-1-RR)

• 

  WB analysis using 87392-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87392-1-RR (PABPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 87392-1-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PABPN1 antibody (87392-1-RR, Clone: 252794F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 87392-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PABPN1 antibody (87392-1-RR, Clone: 252794F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity of 87392-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87382-2-RR against Human PABPN1 were performed. The affinity constant is 22.7 pM.

PABPN1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87392-1-RR

Clone No.252794F12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Nuclear poly(A)-binding protein 1, PAB2, PABII, PABP2, PABP-2

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, SH-SY5Y cells, MCF-7 cells, 4T1 cells, NIH/3T3 cells, PC-12 cells
• Positive IF/ICC detected in: NIH/3T3 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87392-1-RR targets PABPN1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag27814

  Product name: Recombinant human PABPN1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 94-299 aa of BC010939

  Sequence: GSQEEEEEPGLVEGDPGDGAIEDPELEAIKARVREMEEEAEKLKELQNEVEKQMNMSPPPGNAGPVIMSIEEKMEADARSIYVGNVDYGATAEELEAHFHGCGSVNRVTILCDKFSGHPKGFAYIEFSDKESVRTSLALDESLFRGRQIKVIPKRTNRPGISTTDRGFPRARYRARTTNYNSSRSRFYSGFNSRPRGRVYRGRARA

• Full Name: poly(A) binding protein, nuclear 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 50-56 kDa
• GenBank Accession Number: BC010939
• Gene Symbol: PABPN1
• Gene ID (NCBI): 8106
• RRID: AB_3745510
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q86U42
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Poly(A) Binding Protein Nuclear 1(PABPN1) is one of the isoforms of poly(A) binding protein (PABP) that locating to the nucleus in eukaryotic cells. And PABPN1 plays important role in mRNA stability by regulating of poly-A tail length, mRNA decay and proximal polyadenylation site (PAS) utilization at the 3'-UTR (PMID:23300856). Another report showed that PABPN1 shuttles between the nucleus and the cytoplasm to export poly(A) RNA from the nucleus (PMID:23601051). It is reported that PABPN1 is ubiquitously expressed in almost all tissues, however, mutations in PABPN1 causes Oculopharyngeal Muscular Dystrophy (OPMD) which affected in skeletal muscles specifically. Because of a mild reduction in PABPN1 levels is sufficient to induce muscle wasting (PMID:27152426). Acetylation, Methylation, Phosphoprotein are common post-translational modifications of PABPN1 protein. And 50-55 kDa bands have been reported (PMID: 29939290; 28361972).