PACS2 Polyclonal antibody, PBS Only

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• PACS2 Polyclonal antibody, PBS Only (30172-1-PBS)

• 

  WB analysis using 30172-1-AP (same clone as 30172-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30172-1-AP (PACS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30172-1-PBS in a different storage buffer formulation.

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  WB analysis of HCT 116 using 30172-1-AP (same clone as 30172-1-PBS)

  WB result of PACS2 antibody (30172-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACS2 transfected HCT 116 cells. This data was developed using the same antibody clone with 30172-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 30172-1-AP (same clone as 30172-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30172-1-AP (PACS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30172-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HCT 116 using 30172-1-AP (same clone as 30172-1-PBS)

  IP result of anti-PACS2 (IP:30172-1-AP, 4ug; Detection:30172-1-AP 1:1500) with HCT 116 cells lysate 2440 ug. This data was developed using the same antibody clone with 30172-1-PBS in a different storage buffer formulation.

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  IF Staining of HCT 116 using 30172-1-AP (same clone as 30172-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using PACS2 antibody (30172-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30172-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PACS2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 30172-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

30172-1-PBS targets PACS2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag32902

  Product name: Recombinant human PACS2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 340-500 aa of NM_001100913

  Sequence: SARSHKEPPSPADVPEKTRSLGGRQPSDSVSDTVALGVPGPREHPGQPEDSPEAEASTLDVFTERLPPSGRITKTESLVIPSTRSEGKQAGRRGRSTSLKERQAARPQNERANSLDNERCPDARSQLQIPRKTVYDQLNHILISDDQLPENIILVNTSDWQ

• Full Name: phosphofurin acidic cluster sorting protein 2
• Calculated Molecular Weight: 98kd
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: NM_001100913
• Gene Symbol: PACS2
• Gene ID (NCBI): 23241
• RRID: AB_3086254
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86VP3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PACS2, also named PACS1L or KIAA0602, is one of PACS (phosphofurin acidic cluster sorting) proteins which are one such family of multifunctional membrane traffic regulators that mediate organ homoeostasis and have important roles in diverse pathologies and disease states. PACS2 is a multifunctional sorting protein that controls the endoplasmic reticulum (ER)-mitochondria communication, ER homeostasis, and apoptosis. PACS2 may be involved in ion channel trafficking, directing acidic cluster-containing ion channels to distinct subcellular compartments.