PACSIN1 Polyclonal antibody

PACSIN1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13219-1-AP

Synonyms

KIAA1379, PACSIN1, SDPI

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Validation Data Gallery

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  WB analysis of mouse cerebellum using 13219-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13219-1-AP (PACSIN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13219-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13219-1-AP (PACSIN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 13219-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13219-1-AP (PACSIN1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 13219-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13219-1-AP (PACSIN1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human brain using 13219-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13219-1-AP (PACSIN1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 13219-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13219-1-AP (PACSIN1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 13219-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 13219-1-AP (PACSIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SH-SY5Y using 13219-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 13219-1-AP (PACSIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue, mouse brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

13219-1-AP targets PACSIN1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PACSIN1 fusion protein Ag4102
• Full Name: protein kinase C and casein kinase substrate in neurons 1
• Calculated Molecular Weight: 444 aa, 51 kDa
• Observed Molecular Weight: 48-51 kDa
• GenBank Accession Number: BC040228
• Gene Symbol: PACSIN1
• Gene ID (NCBI): 29993
• RRID: AB_10637851
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PACSIN1 (also known as syndapin-1) is a member of the protein kinase C and casein kinase substrate in neurons (PACSIN) family. In mammals, the PACSIN family is comprised of three members, PACSIN1, PACSIN2, and PACSIN3 (PMID: 34990060). PACSIN1 is expressed mainly in neurons, whereas PACSIN2 is ubiquitously expressed in all tissues, and PACSIN3 is expressed mainly in skeletal muscle and the heart (PMID: 23668323). All of these three members contain an N-terminal F-BAR domain and a C-terminal SH3 domain. PACSIN1 plays a role in endocytosis and endosomal recycling. Meanwhile, it has a role in actin remodeling and microtubule nucleation and also plays a particular role in membrane shaping and reconstruction (PMID: 23035120; 34422904). PACSIN1 is involved in neuromorphogenesis and the regulation of the nervous system, and the inappropriate expression of PACSIN1 has been associated with some neurological diseases, including schizophrenia, Alzheimer's disease, and Huntington's disease (PMID: 34990060).

Publications

Species	Application	Title
human	IHC	Front Mol Biosci; Decreased Expression of PACSIN1 in Brain Glioma Samples Predicts Poor Prognosis.; Authors - Zhou Zimu