PACSIN2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PACSIN2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 10518-2-AP

Synonyms

Syndapin-II, Syndapin-2, SdpII, Protein kinase C and casein kinase substrate in neurons protein 2

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 10518-2-AP

  WB result of PACSIN2 antibody (10518-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACSIN2 transfected NIH/3T3 cells.

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  WB analysis using 10518-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10518-2-AP (PACSIN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10518-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10518-2-AP (PACSIN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10518-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10518-2-AP (PACSIN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10518-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10518-2-AP (PACSIN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 10518-2-AP

  IP result of anti-PACSIN2 (IP:10518-2-AP, 4ug; Detection:10518-2-AP 1:500) with NIH/3T3 cells lysate 1200ug.

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  IHC staining of human breast cancer using 10518-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10518-2-AP (PACSIN2 Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human breast cancer using 10518-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10518-2-AP (PACSIN2 Antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human skin using 10518-2-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10518-2-AP (PACSIN2 Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human skin using 10518-2-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10518-2-AP (PACSIN2 Antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human breast cancer using 10518-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10518-2-AP (PACSIN2 antibody) at dilution of 1:100 (under 25x lens).

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  IF Staining of NIH/3T3 using 10518-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 10518-2-AP (PACSIN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse heart tissue, mouse lung tissue, NIH/3T3 cells, HepG2 cells
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human breast cancer tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IF: See 5 publications below

Product Information

10518-2-AP targets PACSIN2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PACSIN2 fusion protein Ag0809
• Full Name: protein kinase C and casein kinase substrate in neurons 2
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC008037
• Gene Symbol: PACSIN2
• Gene ID (NCBI): 11252
• RRID: AB_2161854
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peripheral membrane proteins of the Bin/amphiphysin/Rvs (BAR) and Fer-CIP4 homology-BAR (F-BAR) family participate in cellular membrane trafficking (PMID: 19549836). PACSIN2 (also known as syndapin-2) is a member of the protein kinase C and casein kinase substrate in neurons (PACSIN) family, which are membrane-binding proteins characterized by an amino-terminal F-BAR domain. PACSIN2 plays a role in intracellular vesicle-mediated transport. It is involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization (PMID: 23129763). PACSIN2 also has a role in caveolae membrane sculpting (PMID: 21610094).

Publications

Species	Application	Title
human	WB,IF	Mol Biol Cell; Cooperation of MICAL-L1, syndapin2, and phosphatidic acid in tubular recycling endosome biogenesis.; Authors - Giridharan Sai Srinivas Panapakkam SS ; KD Validated
human	WB	PLoS One; Role of Phosphatidylinositol 4,5-Bisphosphate in Regulating EHD2 Plasma Membrane Localization.; Authors - Simone Laura C LC ; KD Validated
human	WB,IF	J Bacteriol; Application of a C. trachomatis expression system to identify C. pneumoniae proteins translocated into host cells.; Authors - Izumi Yanatori
human	WB	Cell Rep; Endogenous Cyclin D1 Promotes the Rate of Onset and Magnitude of Mitogenic Signaling via Akt1 Ser473 Phosphorylation.; Authors - Ke Chen
human,mouse,zebrafish	WB,IF	Nat Commun; Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport.; Authors - Christine Insinna ; KO Validated
human	WB	J Dent Res; Proteome Analysis of Temporomandibular Joint with Disc Displacement; Authors - X Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Herve (Verified Customer) (11-13-2019)

This antibody recognizes a 55-kDa band that is present in both Pacsin2 WT and KO platelet lysates. PACSIN2 runs normally at 65 kDa in human and mouse platelet lysates and is absent in mouse Pacsin2 KO samples.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Platelets