Tested Applications
| Positive WB detected in | mouse brain tissue, MCF-7 cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue, human breast cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12110-1-AP targets PADI2 in WB, IHC, IF-P, IP, CoIP, ELISA, Blocking assay applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, canine, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2755 Product name: Recombinant human PADI2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 118-438 aa of BC009701 Sequence: ISLDVDADRDGVVEKNNPKKASWTWGPEGQGAILLVNCDRETPWLPKEDCRDEKVYSKEDLKDMSQMILRTKGPDRLPAGYEIVLYISMSDSDKVGVFYVENPFFGQRYIHILGRRKLYHVVKYTGGSAELLFFVEGLCFPDEGFSGLVSIHVSLLEYMAQDIPLTPIFTDTVIFRIAPWIMTPNILPPVSVFVCCMKDNYLFLKEVKNLVEKTNCELKVCFQYLNRGDRWIQDEIEFGYIEAPHKGFPVVLDSPRDGNLKDFPVKELLGPDFGYVTREPLFESVTSLDSFGNLEVSPPVTVNGKTYPLGRILIGSSFPL Predict reactive species |
| Full Name | peptidyl arginine deiminase, type II |
| Calculated Molecular Weight | 665 aa, 75 kDa |
| Observed Molecular Weight | 70-75 kDa, 50 kDa |
| GenBank Accession Number | BC009701 |
| Gene Symbol | PADI2 |
| Gene ID (NCBI) | 11240 |
| RRID | AB_2159475 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2J8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PADI2, also named as KIAA0994, PDI2, PAD-H19, and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation-related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PADI2 antibody 12110-1-AP | Download protocol |
| IHC protocol for PADI2 antibody 12110-1-AP | Download protocol |
| IP protocol for PADI2 antibody 12110-1-AP | Download protocol |
| WB protocol for PADI2 antibody 12110-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-PADI2 antibody (Cat# 12110-1-AP) has 80 total citations. Publications appear in journals including Immunity, Nature Neuroscience, Nature Communications, Cancer Research, PNAS, Cell Reports, Science Translational Medicine, JCI Insight, ACS Chemical Biology, and Frontiers in Immunology. Research fields span oncology (prostate, breast, colorectal, lung, oral), neurology (Alzheimer's, myelination, spinal cord injury), immunology (NETs, sepsis, lupus, rheumatoid arthritis), reproductive biology, cardiovascular disease, and dermatology.
| Species | Application | Title |
|---|---|---|
Immunity Astrocytic calcium-dependent enzyme PAD2 governs microglia activity to exacerbate amyloid pathology via citrullinated vimentin | ||
Cancer Res PAD2 Overexpression in Transgenic Mice Promotes Spontaneous Skin Neoplasia.
| ||
Cancer Res PADI2-mediated citrullination promotes prostate cancer progression.
| ||
Nat Neurosci Derivation and transcriptional reprogramming of border-forming wound repair astrocytes after spinal cord injury or stroke in mice | ||
Nat Commun A citrullinated histone H3 monoclonal antibody for immune modulation in sepsis. | ||
Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot and cell culture application. The reviewer notes that their lab uses several different antibodies across multiple projects, indicating consistent, reliable performance in their workflow. Overall, the feedback is positive, reflecting satisfaction with the product's quality and utility in routine research.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Michael (Verified Customer) (07-23-2025) | This lab uses several different antibodies across multiple projects.
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