PADI4 Recombinant monoclonal antibody

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• PADI4 Recombinant monoclonal antibody (86419-1-RR)

• 

  WB analysis using 86419-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86419-1-RR (PADI4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 86419-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using PADI4 antibody (86419-1-RR, Clone: 250881C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

PADI4 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86419-1-RR

Clone No.250881C10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

EC:3.5.3.15, HL 60 PAD, HL-60 PAD, PAD, PAD4

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, mouse spleen tissue, rat spleen tissue
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86419-1-RR targets PADI4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag11339

  Product name: Recombinant human PADI4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 317-663 aa of BC025718

  Sequence: EDFLKSVTTLAMKAKCKLTICPEEENMDDQWMQDEMEIGYIQAPHKTLPVVFDSPRNRGLKEFPIKRVMGPDFGYVTRGPQTGGISGLDSFGNLEVSPPVTVRGKEYPLGRILFGDSCYPSNDSRQMHQALQDFLSAQQVQAPVKLYSDWLSVGHVDEFLSFVPAPDRKGFRLLLASPRSCYKLFQEQQNEGHGEALLFEGIKKKKQQKIKNILSNKTLREHNSFVERCIDWNRELLKRELGLAESDIIDIPQLFKLKEFSKAEAFFPNMVNMLVLGKHLGIPKPFGPVINGRCCLEEKVCSLLEPLGLQCTFINDFFTYHIRHGEVHCGTNVRRKPFSFKWWNMVP

• Full Name: peptidyl arginine deiminase, type IV
• Calculated Molecular Weight: 663 aa, 74 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC025718
• Gene Symbol: PADI4
• Gene ID (NCBI): 23569
• RRID: AB_3744867
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UM07
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PADI4, also named PADI5 and PDI5, catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. PADI4 is expressed in hematologic and rheumatoid arthritis synovial tissues. PADI4 haplotype associated with susceptibility to rheumatoid arthritis increases the production of citrullinated peptides, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Immunohistochemistry detected significant PADI4 expression in various malignancies and PADI4 expression was not detected in non-tumor tissues. (PMID: 19183436)