PAF1 Polyclonal antibody

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• KD/KO Validated

PAF1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 15441-1-AP Copied!

Publications (10)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

F23149_1, hPAF1, PAF1, PD2

• 

  WB analysis of HepG2 using 15441-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15441-1-AP (PAF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 15441-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15441-1-AP (PAF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 15441-1-AP

  IP result of anti-PAF1 (IP:15441-1-AP, 3ug; Detection:15441-1-AP 1:1000) with HeLa cells lysate 3000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 15441-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15441-1-AP (PAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 15441-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15441-1-AP (PAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 15441-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15441-1-AP (PAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 15441-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15441-1-AP (PAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 15441-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15441-1-AP (PAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 15441-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15441-1-AP (PAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary tumor tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15441-1-AP targets PAF1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7212

  Product name: Recombinant human PAF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 166-531 aa of BC000017

  Sequence: EEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMDYAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRAKAGVQSGTNALLVVKHRDMNEKELEAQEARKAQLENHEPEEEEEEEMETEEKEAGGSDEEQEKGSSSEKEGSEDEHSGSESEREEGDRDEASDKSGSGEDESSEDEARAARDKEEIFGSDADSEDDADSDDEDRGQAQGGSDNDSDSGSNGGGQRSRSHSRSASPFPSGSEHSAQEDGSEAAASDSSEADSDSD

• Full Name: Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC000017
• Gene Symbol: PAF1
• Gene ID (NCBI): 54623
• RRID: AB_2174457
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N7H5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAF1, also known as hPAF1 or PD2, is a PAF (RNA Polymerase II Associated Factor) complex subunit. Paf1 functions primarily to modulates transcript elongation. PAF1 occupies transcriptional enhancers and restrains hyperactivation of a subset of these enhancers(PMID: 28860207). The PAF complex also has a role in histone monoubiquitination. Paf1 is overexpressed in pancreatic cancer cells and has demonstrated potential oncogenic property. The MW of this protein is 80 kDa, and this antibody specially recognises the 80 kDa protein.

Publications

What published studies show

PAF1 antibody (Cat# 15441-1-AP) has 10 total citations published in journals including Cell, Autophagy, Nucleic Acids Research, Science Advances, Cell Insight, J Cell Biology, J Cell Mol Med, J Biol Chem, ACS Pharmacol Transl Sci, and Radiat Oncol. Associated research fields span virology, mitophagy, DNA damage repair, transcription elongation, chemoresistance, phase separation, colorectal cancer, lung cancer, tumor cell cycle regulation, and radiosensitivity.

Automatically generated from the 10 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; SARS-CoV-2 variants evolve convergent strategies to remodel the host response; Authors - Mehdi Bouhaddou
human	WB	Autophagy; The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.; Authors - Liangde Zheng ; KD Validated
human		Nucleic Acids Res; The dimeric deubiquitinase USP28 integrates 53BP1 and MYC functions to limit DNA damage; Authors - Chao Jin
human	WB	Sci Adv; CDK12 and Integrator-PP2A complex modulates LEO1 phosphorylation for processive transcription elongation; Authors - Min Qiu
human	WB	Cell Insight; PAF1c depletion confers chemoresistance to topoisomerase inhibitors.; Authors - Dingwei Li
human	WB	J Cell Biol; Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine.; Authors - Chen Chen