PAFAH1B3 Polyclonal antibody

• Featured Product
• KD/KO Validated

PAFAH1B3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 20564-1-AP

Synonyms

Platelet-activating factor acetylhydrolase IB subunit alpha1, PAFAHG, PAF-AH subunit gamma, PAF-AH 29 kDa subunit, EC:3.1.1.47

---

Validation Data Gallery

• 

  WB analysis of human brain using 20564-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20564-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20564-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 20564-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 20564-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 20564-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: human brain tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

20564-1-AP targets PAFAH1B3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: platelet-activating factor acetylhydrolase, isoform Ib, gamma subunit 29kDa
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26-29 kDa
• GenBank Accession Number: NM_002573
• Gene Symbol: PAFAH1B3
• Gene ID (NCBI): 5050
• RRID: AB_10693534
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAFAH1B3, also named as PAFAHG, belongs to the 'GDSL' lipolytic enzyme family and Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily. It inactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. PAFAH1B3 plays an important role during the development of brain. The antibody is specific to PAFAH1B3.

Publications

Species	Application	Title
human	WB,IHC	Onco Targets Ther; Aberrant expression of PAFAH1B3 associates with poor prognosis and affects proliferation and aggressiveness in hypopharyngeal squamous cell carcinoma.; Authors - Jianing Xu ; KD Validated
human	IHC,IF	Discov Oncol; Increased PAFAH1B3 was associated with poor prognosis and T-cell exhaustion microenvironment in hepatocellular carcinoma; Authors - Genhao Zhang
human	IHC	Discov Oncol; Prognostic clinical phenotypes associated with tumor stemness in the immune microenvironment of T-cell exhaustion for hepatocellular carcinoma; Authors - Genhao Zhang