PAG1 Polyclonal antibody

PAG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25029-1-AP

Synonyms

Phosphoprotein associated with glycosphingolipid-enriched microdomains 1, PAG, Csk-binding protein, Csk binding protein, CBP

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 25029-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25029-1-AP (PAG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 25029-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25029-1-AP (PAG1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25029-1-AP targets PAG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAG1 fusion protein Ag13393
• Full Name: phosphoprotein associated with glycosphingolipid microdomains 1
• Calculated Molecular Weight: 432 aa, 47 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC112159
• Gene Symbol: PAG1
• Gene ID (NCBI): 55824
• RRID: AB_2879858
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAG1, also named as CBP and PAG, promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. It is involved in cell adhesion signaling. PAG1 may play an important role in inhibiting the proliferation, invasion and metastasis of the cancer cells. PAG1 is a membrane protein with phosphor modification. The MW of PAG1 migrates to 50-65 kDa in WB detection.