PAIP2 Polyclonal antibody

PAIP2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15583-1-AP

Synonyms

PAIP2A, PAIP-2, PAIP 2, PABP-interacting protein 2, PABP interacting protein 2

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Validation Data Gallery

• 

  WB analysis of A431 using 15583-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15583-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15583-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15583-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15583-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 15583-1-AP

  IP result of anti-PAIP2 (IP:15583-1-AP, 4ug; Detection:15583-1-AP 1:500) with A431 cells lysate 1200ug.

• 

  IF Staining of HeLa using 15583-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15583-1-AP (PAIP2 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, Jurkat cells, mouse lung tissue
• Positive IP detected in: A431 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

15583-1-AP targets PAIP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAIP2 fusion protein Ag7119
• Full Name: poly(A) binding protein interacting protein 2
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC001716
• Gene Symbol: PAIP2
• Gene ID (NCBI): 51247
• RRID: AB_10950891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IHC	J Neurosci; Proteasome modulates positive and negative translational regulators in long-term synaptic plasticity.; Authors - Chenghai Dong
human	WB	Front Genet; PAIP2 is a potential diagnostic and prognostic biomarker of breast cancer and is associated with immune infiltration; Authors - Chenyu Wang
mouse	IP	Pharmacol Res; Inhibition of K63 ubiquitination by G-Protein pathway suppressor 2 (GPS2) regulates mitochondria-associated translation; Authors - Yuan Gao