Tested Applications
| Positive WB detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16071-1-AP targets PAK1IP1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9016 Product name: Recombinant human PAK1IP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-392 aa of BC010907 Sequence: MELVAGCYEQVLFGFAVHPEPEACGDHEQWTLVADFTHHAHTASLSAVAVNSRFVVTGSKDETIHIYDMKKKIEHGALVHHSGTITCLKFYGNRHLISGAEDGLICIWDAKKWECLKSIKAHKGQVTFLSIHPSGKLALSVGTDKTLRTWNLVEGRSAFIKNIKQNAHIVEWSPRGEQYVVIIQNKIDIYQLDTASISGTVTNEKRISSVKFLSESVLAVAGDEEVIRFFDCDSLVCLCEFKAHENRVKDMFSFEIPEHHVIVSASSDGFIKMWKLEQDKKVPPSLLCEINTNARLTCLGVWLDKVADMKESLPPAAEPSPVSKEQSKIGKKEPGDTVHKEEKRSKPNTKKRGLTGDSKKATKESGLISTKKRKMVEMLEKKRKKKKIKTMQ Predict reactive species |
| Full Name | PAK1 interacting protein 1 |
| Calculated Molecular Weight | 392 aa, 44 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC010907 |
| Gene Symbol | PAK1IP1 |
| Gene ID (NCBI) | 55003 |
| RRID | AB_2160561 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NWT1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PAK1IP1 antibody 16071-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PAK1IP1 Polyclonal antibody (Cat# 16071-1-AP) has 1 citation to date. The cited publication appeared in Frontiers in Bioscience (Landmark Edition) (2025), with an impact factor of 4.1. The research field is hepatocellular carcinoma, specifically investigating how knockdown of PAK1IP1 induces pyroptosis to inhibit tumor progression. The antibody was used in Western blot (WB) on human samples, validated by RNAi knockdown.
| Species | Application | Title |
|---|---|---|
Front Biosci (Landmark Ed) Knockdown of PAK1IP1 can Induce Pyroptosis to Inhibit the Progression of Hepatocellular Carcinoma
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